Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression
Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression
复制标题
桑树 EIL3 赋予盐和干旱耐受性并调节乙烯生物合成基因表达
DOI：
10.7717/peerj.6391
复制
发表时间：
2019-02-19
期刊：
影响因子：
2.7
通讯作者：
Zhao, Aichun
中科院分区：
文献类型：
--
作者：
Liu, Changying;Li, Jun;Zhao, Aichun
乙烯调节植物对非生物胁迫的响应和耐受性，乙烯不敏感3（EIN3）/类EIN3（EIL）蛋白是乙烯信号转导的关键组分。尽管在模式植物中已经研究了EIN3/EIL蛋白在应对非生物胁迫方面的功能，但在非模式植物中知之甚少，包括桑树（桑属），它是一种具有重要经济价值的多年生木本植物。我们对一个来自桑树编码类EIN3蛋白的基因进行了功能鉴定，将其命名为MnEIL3。实时定量PCR分析表明，盐胁迫和干旱胁迫可诱导MnEIL3在根和茎中的表达。过表达MnEIL3的拟南芥对盐胁迫和干旱胁迫表现出更强的耐受性。在拟南芥中过表达MnEIL3显著上调了乙烯生物合成基因的转录丰度。此外，MnEIL3增强了对盐胁迫和干旱胁迫有响应的MnACO1和MnACS1启动子的活性。因此，MnEIL3可能在对非生物胁迫的耐受性以及乙烯生物合成基因的表达中发挥重要作用。
Ethylene regulates plant abiotic stress responses and tolerances, and ethylene-insensitive3 (EIN3)/EIN3-like (EIL) proteins are the key components of ethylene signal transduction. Although the functions of EIN3/EIL proteins in response to abiotic stresses have been investigated in model plants, little is known in non-model plants, including mulberry (Morus L.), which is an economically important perennial woody plant. We functionally characterized a gene encoding an EIN3-like protein from mulberry, designated as MnEIL3. A quantitative real-time PCR analysis demonstrated that the expression of MnEIL3 could be induced in roots and shoot by salt and drought stresses. Arabidopsis overexpressing MnEIL3 exhibited an enhanced tolerance to salt and drought stresses. MnEIL3 overexpression in Arabidopsis significantly upregulated the transcript abundances of ethylene biosynthetic genes. Furthermore, MnEIL3 enhanced the activities of the MnACO1 and MnACS1 promoters, which respond to salt and drought stresses. Thus, MnEIL3 may play important roles in tolerance to abiotic stresses and the expression of ethylene biosynthetic genes.