Organelle zones.
Organelle zones.
复制标题
细胞器区。
DOI：
10.1091/mbc.e18-12-0818
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发表时间：
2019
影响因子：
3.3
通讯作者：
vonBlume,Julia
中科院分区：
文献类型：
--
作者：
Nakano,Akihiko;vonBlume,Julia
“细胞器区”Minisymphilosophy侧重于细胞器内小区域功能分化的新概念。活细胞成像的时空分辨率的最新改进，使细胞器中的小区域，我们建议称之为“细胞器区”的检测。细胞器区可分类为1）响应于应激而出现的响应区，2）在细胞器之间的接触位点处限定的通信区，和3）存在于运输细胞器（例如内质网（ER）和高尔基体）中的分选区。此外，还进行了与在该领域具有重要意义的显微镜尖端技术相关的讲座。中野彦（日本和子理研中心）作为共同主持人介绍了细胞器带的概念，并介绍了为对细胞器带和囊泡的行为进行高速成像而开发的超分辨率共聚焦实时成像显微镜（SCLIM）。另一位共同主席Julia von Blume（德国慕尼黑马克斯-普朗克生物化学研究所）谈到了跨高尔基体网络（TGN）中可溶性分泌物的分选。关键的参与者是Cab 45，它在与SPCA 1泵吸收的Ca 2+结合后寡聚化。Cab 45将分泌性货物包装到富含鞘磷脂的TGN衍生的囊泡载体中，其在TGN中的合成因此将Ca 2+通量和货物分选偶联（www. ncbi. nlm。nih。gov/pubmed/30393074）。
The “Organelle Zones” Minisymposium was focused on new concepts in functional differentiation of small regions within organelles. Recent improvements in spatiotemporal resolution of live cell imaging has enabled detection of small regions in organelles, which we propose to call “organelle zones.” Organelle zones may be categorized as 1) response zones that appear in response to stress, 2) communication zones defined at the contact sites between organelles, and 3) sorting zones present in trafficking organelles such as the endoplasmic reticulum (ER) and the Golgi. Several talks were related to cutting-edge technologies of microscopy, which is very important in this field.Akihiko Nakano (RIKEN Center for Advanced Photonics, Wako, Japan) as a cochair introduced the concept of organelle zones and presented his efforts to develop superresolution confocal live imaging microscopy (SCLIM) for high-speed imaging of behaviors of small organelle zones and vesicles. Julia von Blume (Max-Planck Institute for Biochemistry, Munich, Germany), the other cochair, talked about the sorting of soluble secretory cargo in the trans-Golgi network (TGN). The key player is Cab45, which is oligomerized upon binding to Ca2+ taken up by the SPCA1 pump. Cab45 packages secretory cargo into a TGN-derived vesicular carrier enriched in sphingomyelin, whose synthesis in TGN thus couples Ca2+ flux and cargo sorting (www. ncbi. nlm. nih. gov/pubmed/30393074).