Immunomodulatory effects of hyperthermia on resisting WSSV infection in Procambarus clarkii.
Immunomodulatory effects of hyperthermia on resisting WSSV infection in Procambarus clarkii.
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DOI：
10.1111/jfd.12268
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发表时间：
2015-06
影响因子：
2.5
通讯作者：
X. Wu;Haitao Xiong;Yu Wang;Huahua Du
中科院分区：
文献类型：
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作者：
X. Wu;Haitao Xiong;Yu Wang;Huahua Du
白色斑点病仍然是世界范围内水产养殖业的一个持续威胁。热疗已被证明可以降低白色斑点综合征病毒（WSSV）感染虾的死亡率，但其机制仍不清楚。在这项研究中，我们试图确定宿主的免疫因素，有助于抑制WSSV感染在高温。在24 ± 1 °C培养的WSSV感染的克氏原螯虾（Procambarus clarkii（吉拉德）中，热休克蛋白70（Hsp70）基因的转录水平在感染后48小时（hpi）后在血细胞中显示出适度的2.2倍的增加。相比之下，在32 ± 1 °C下培养的WSSV感染的小龙虾中，Hsp70基因的表达显示出快速的诱导，在4hpi时为19.5倍。这表明热休克蛋白70在热疗抵抗WSSV感染中起着积极的调节作用。此外，总血细胞计数（THC）和酚氧化酶（PO）的活性都显着增加在WSSV感染的螯虾在32 ± 1 °C培养48 hpi。这两个可能是至关重要的小龙虾生存在后期阶段的WSSV感染。总体而言，上调宿主蛋白Hsp70的表达以及增加THC和PO活性表明，高温具有免疫调节作用，增强了克氏疟原虫对WSSV感染的抵抗力。
White spot disease remains a constant threat to aquaculture worldwide. Hyperthermia has been shown to reduce mortality in white spot syndrome virus (WSSV)-infected shrimps, but the mechanism still remains unclear. In this study, we sought to identify host immune factors that contribute to inhibition of WSSV infection during hyperthermia. In WSSV-infected red swamp crayfish Procambarus clarkii (Girard) cultured at 24 ± 1 °C, transcriptional levels of the heat shock protein 70 (Hsp70) gene showed a modest, 2.2-fold increase in haemocytes following 48 h post-infection (hpi). In contrast, in WSSV-infected crayfish cultured at 32 ± 1 °C, Hsp70 gene expression showed a rapid, 19.5-fold induction by 4 hpi. This suggests that Hsp70 plays a positive regulatory role in resistance to WSSV infection during hyperthermia. Furthermore, total haemocyte counts (THC) and phenoloxidase (PO) activity were both significantly increased in WSSV-infected crayfish cultured at 32 ± 1 °C by 48 hpi. Both may be critical for crayfish survival in the late stages of WSSV infection. Collectively, the up-regulation of host protein Hsp70 expression and increase in THC and PO activity suggest that hyperthermia has immunomodulatory effect that enhanced the resistance of P. clarkii to WSSV infection.