Induction of defense-related enzymes in soybean leaves by class IId bacteriocins (thuricin 17 and bacthuricin F4) purified from Bacillus strains
Induction of defense-related enzymes in soybean leaves by class IId bacteriocins (thuricin 17 and bacthuricin F4) purified from Bacillus strains
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DOI：
10.1016/j.micres.2011.02.004
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发表时间：
2011-01-01
影响因子：
6.7
通讯作者：
Smith, Donald L.
中科院分区：
文献类型：
--
作者：
Jung, Woo-Jin;Mabood, Fazli;Smith, Donald L.
我们最近发现了一类新的细菌素（IId 类），它以类似于 Nod 因子的方式刺激植物生长。点头因子已被证明可以激发植物抗病性的各个方面。我们研究了细菌素 [thricin 17 (T17) 和 bacthuricin F4 (BF4)] 对苯丙氨酸解氨酶 (PAL)、愈创木酚过氧化物酶 (POD)、抗坏血酸过氧化物酶 (APX)、超氧化物歧化酶 (SOD) 和多酚氧化酶 (PPO) 活性的影响。在第一个三叶阶段，将细菌素溶液喂入大豆（Glycine max L. Merr. cv. OAC Bayfield）幼苗的切茎中。 T17处理叶片中PAL活性在处理后72小时达到最高，比当时的对照高75.5%。处理后72小时，与对照处理相比，117和BF4处理叶片的POD活性分别增加了72.7%和91.3%。处理后72小时，T17和BF4处理叶片的APX活性分别为52.3%和49.6%，高于对照。 T17处理叶片SOD活性在处理后72小时最高，比此时对照高26.0%。 72 小时时，117 和 BF4 处理的叶片中 SOD 活性分别比对照高 70.5% 和 60.2%。使用 PAGE，我们发现一种 APX 同工酶（28 kDa 同工型）在所有细菌素处理的叶子中在 72 小时时表现出最强的诱导作用。相对于对照处理，两种细菌素均增加了七种 SOD 同工酶的活性。相对于对照处理，两种细菌素均强烈诱导 33 kDa PPO 同工酶。这些结果表明，类盖细菌素可以充当植物病害防御相关酶的诱导剂，并且可能通过类似于 Nod 因子的机制发挥作用。 (C) 2011 爱思唯尔有限公司。版权所有。
We have recently discovered a new class of bacteriocin (class IId) which stimulates plant growth in a way similar to Nod factors. Nod factors have been shown to provoke aspects of plant disease resistance. We investigated the effects of bacteriocins [thuricin 17 (T17) and bacthuricin F4 (BF4)] on the activities of phenylalanine ammonia lyase (PAL), guaiacol peroxidase (POD), ascorbate peroxidase (APX), superoxide dismutase (SOD), and polyphenol oxidase (PPO). Bacteriocin solutions were fed into the cut stems of soybean (Glycine max L. Merr. cv. OAC Bayfield) seedlings at the first trifoliate stage. PAL activity in T17 treated leaves was the highest at 72 h after treatment and was 75.5% greater than the control at that time. At 72h after treatment POD activities in 117 and BF4 treated leaves increased by 72.7 and 91.3%, respectively, as compared with the control treatment. APX activity was 52.3 and 49.6% respectively, greater than the control in T17 and BF4 treated leaves at 72 h after treatment. SOD activity in T17 treated leaves was the highest at 72 h after treatment and was 26.0% greater than the control at that time. SOD activity was 70.5 and 60.2% greater, respectively, than the control in 117 and BF4 treated leaves, at 72 h. Using PAGE we found that one APX isozyme (28 kDa isoform) showed the strongest induction in all bacteriocin treated leaves at 72 h. Activity of the seven SOD isozymes was increased by both bacteriocins, relative to the control treatment. The 33 kDa PPO isozyme was induced strongly by both bacteriocins, relative to the control treatment. These results indicate that class lid bacteriocins can act as an inducer of plant disease defense-related enzymes and may be acting through mechanisms similar to Nod factors. (C) 2011 Elsevier GmbH. All rights reserved.