Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor
Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor
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DOI：
10.1021/ja803429z
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发表时间：
2008-09-24
影响因子：
15
通讯作者：
Yamamoto, Yukio
中科院分区：
文献类型：
--
作者：
Taki, Masayasu;Desaki, Mika;Yamamoto, Yukio
本文介绍了一种基于香豆素的镉双激发比率探针CadMQ。这种荧光传感器具有高的量子产率为0.59和0.70的金属自由和Cd2+结合的形式，分别，并具有0.16 nM的Cd2+的解离常数。CadMQ是细胞可渗透的，位于细胞的酸性区室内。我们进一步表明，CadMQ是一个有用的工具，以比例探测细胞内镉2+水平的变化与使用两个激发波长。
We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd2+ bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd2+. CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd2+ levels with the use of two excited wavelengths.