Caenorhabditis elegans prom-1 is required for meiotic prophase progression and homologous chromosome pairing
Caenorhabditis elegans prom-1 is required for meiotic prophase progression and homologous chromosome pairing
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DOI：
10.1091/mbc.e07-03-0243
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发表时间：
2007-12-01
影响因子：
3.3
通讯作者：
Loidl, Josef
中科院分区：
文献类型：
--
作者：
Jantsch, Verena;Tang, Lois;Loidl, Josef
在秀丽线虫突变株中分离到一个新的基因prom-1，该突变株的生殖系中的细胞凋亡率增加。PROM-1编码一种F-box蛋白，与推测的人类肿瘤抑制基因FBXO47同源性有限。PROM-1基因的突变导致二价形成的强烈减少，从而导致胚胎致死率增加和HIM表型。此外，减数分裂前期出现了迟缓和不同步的核重组以及减少的同源突触。重组蛋白RAD-51在减数分裂核中的积累表明双链DNA断裂的修复受到干扰。在PROM-1突变性腺中，细胞核适时完成有丝分裂增殖和减数分裂前复制，但在减数分裂进入时经历了较长的延迟。因此，我们认为PROM-1通过减数分裂前期I调节适时的进程，如果没有PROM-1，同源染色体的识别就会严重受损。
A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in increased embryonic lethality and a Him phenotype. Furthermore, retarded and asynchronous nuclear reorganization as well as reduced homologous synapsis occur during meiotic prophase. Accumulation of recombination protein RAD-51 in meiotic nuclei suggests disturbed repair of double-stranded DNA breaks. Nuclei in prom-1 mutant gonads timely complete mitotic proliferation and premeiotic replication, but they undergo prolonged delay upon meiotic entry. We, therefore, propose that prom-1 regulates the timely progression through meiotic prophase I and that in its absence the recognition of homologous chromosomes is strongly impaired.