Colorimetric analysis of lipopolysaccharides based on its self-assembly to inhibit ion transport
Colorimetric analysis of lipopolysaccharides based on its self-assembly to inhibit ion transport
复制标题
基于脂多糖自组装抑制离子传输的比色分析
DOI：
10.1016/j.aca.2017.09.002
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发表时间：
2017
影响因子：
6.2
通讯作者：
Zhang Juan
中科院分区：
文献类型：
--
作者：
Li Defeng;Sun Tuanwei;Zhang Wenjun;Shen Zhongming;Zhang Juan
A new strategy is proposed based on inhibition of ion transport by lipid bilayer derived from spontaneous assembly of lipopolysaccharides (LPS), thereby a colorimetric method is established for analysis of LPS. At acidic pH values, LPS can specially bind with aminophenylboronic acid modified assembled magnetic nanospheres (APBA/AMNSs), resulting in formation of lipid bilayer around APBA/AMNSs. Under acidic condition, the lipid bilayer can inhibit the release of iron ions from AMNSs into the solution so as to decrease the oxidized extent of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt mediated by hydrogen peroxide. Using the established method, LPS can be detected over the wide linear detection range with the low detection limit. With good selectivity, reproductivity, and simplicity, the method is accurate in LPS tests of real drinking samples.
