eyeless/Pax6 Controls the Production of Glial Cells in the Visual Center of Drosophila melanogaster.
eyeless/Pax6 Controls the Production of Glial Cells in the Visual Center of Drosophila melanogaster.
复制标题
eyeless/Pax6 控制果蝇视觉中心神经胶质细胞的产生。
DOI：
10.1016/j.ydbio.2015.12.004
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发表时间：
2016
影响因子：
2.7
通讯作者：
M.
中科院分区：
文献类型：
--
作者：
Suzuki;T.;Takayama;R. and Sato;M.
Pax6被认为是中枢神经系统发育过程中的神经源性因子，调节神经前体细胞的增殖，促进神经元分化。除了神经发生，Pax6还参与神经胶质细胞的规范和成熟。在这里，我们展示了盲目(Ey)，果蝇Pax6的同源物，调节大脑中神经胶质细胞的产生。在发育中的苍蝇视觉中枢，神经元和神经胶质细胞的产生受时间转录因子的控制，这些转录因子依次在神经母细胞(NBS)中表达。其中，最后一个时间窗的NBS产生星形胶质细胞样细胞。EY在中年神经母细胞瘤中表达较强，其时间窗比神经胶质细胞产生老年神经母细胞瘤的时间更早。在产生NBS的神经胶质细胞中也检测到微弱的Ey表达。我们的结果表明，Ey在中年NBS中的表达通过调节其他时间转录因子来间接控制最古老的NBS的胶质形成。此外，在最后一个时间窗的NBS中弱的Ey表达可能直接控制神经胶质的发生。EY也在EY阳性的时间窗的NBS产生的神经元中表达。有趣的是，神经元特异性的Ey过表达导致胶质细胞显著增加，这表明神经元Ey的表达也可能有助于神经胶质的发生。因此，依赖于Pax6的星形胶质细胞样胶质细胞发育的调控在整个动物界都是保守的。
Pax6 is known as a neurogenic factor in the development of the central nervous system and regulates proliferation of neuronal progenitor cells and promotes neuronal differentiation. In addition to neurogenesis, Pax6 is also involved in the specification and maturation of glial cells. Here, we show that Eyeless (Ey),Drosophilahomolog of Pax6, regulates the production of glial cells in the brain. In the developing fly visual center, the production of neurons and glial cells are controlled by the temporal transcription factors that are sequentially expressed in neuroblasts (NBs). Among them, NBs of the last temporal window produce astrocyte-like glial cells. Ey is strongly expressed in the middle aged NBs, whose temporal window is earlier compared with glia producing older NBs. Weak Ey expression is also detected in the glia producing NBs. Our results suggest that Ey expression in the middle aged NBs indirectly control gliogenesis from the oldest NBs by regulating other temporal transcription factors. Additionally, weak Ey expression in the NBs of last temporal window may directly control gliogenesis. Ey is also expressed in neurons produced from the NBs of Ey-positive temporal window. Interestingly, neuron-specific overexpression of Ey causes significant increase in glial cells suggesting that neuronal expression of Ey may also contribute to gliogenesis. Thus, Pax6-dependent regulation of astrocyte-like glial development is conserved throughout the animal kingdom.