Production, circulation, and excretion of melanin-related metabolites in B16 melanoma-bearing mice.
Production, circulation, and excretion of melanin-related metabolites in B16 melanoma-bearing mice.
复制标题
B16 黑色素瘤小鼠体内黑色素相关代谢物的产生、循环和排泄。
DOI：
10.2340/0001555570367372
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发表时间：
1990
影响因子：
3.6
通讯作者：
K. Fujita
中科院分区：
文献类型：
--
作者：
K. Wakamatsu;S. Ito;K. Fujita
尿中5-S-半胱氨酸多巴(5-S-CD)已被用作黑色素瘤转移的生化标志物。建立了测定小容量血清中真黑素代谢产物5(6)-羟基-6(5)-甲氧基吲哚-2-羧酸(5H6MI2C和6H5MI2C)的方法。我们比较了这些吲哚和5-S-CD在黑色素瘤中的产生、血液循环和尿液排泄与高度色素的B16小鼠黑色素瘤的重量的相关性。5H6MI2C+6H5MI2C(r=0.92)和5-S-CD(r=0.89)血药浓度与肿瘤重量呈正相关。而5H6MI2C+6H5MI2C与5-S-CD的尿排出量无显著相关性。提示血清5H6MI2C+6H5MI2C和5-S-CD比尿液更能反映黑色素瘤的进展情况。此外，对色素含量高和色素较少的B16黑色素瘤的黑色素相关代谢产物含量的比较表明，5-S-CD可能通过与黑色素结合而在色素黑色素瘤中积聚，儿茶酚-O-甲基转移酶可能在色素沉着中起到调节作用。
Urinary 5-S-cysteinyldopa (5-S-CD) has been used as a biochemical marker of melanoma metastasis. A method was developed for determining the eumelanin-related metabolites 5(6)-hydroxy-6(5)-methyoxyindole-2-carboxylic acids (5H6MI2C and 6H5MI2C) in small volumes of serum. We compared these indoles and 5-S-CD regarding the correlation of their production in melanoma, circulation in blood, and excretion in urine, with the weight of highly pigmented, B16 mouse melanoma. An excellent correlation was found between the serum concentration of 5H6MI2C + 6H5MI2C (r = 0.92) and 5-S-CD (r = 0.89) and tumor weight. However, the urinary excretion of 5H6MI2C + 6H5MI2C and 5-S-CD did not show any significant correlation. These results suggest that 5H6MI2C + 6H5MI2C and 5-S-CD in serum may better reflect melanoma progression than those in urine. Furthermore, comparison of the contents of these melanin-related metabolites between highly pigmented and less pigmented B16 melanomas suggests that 5-S-CD may be accumulated in pigmented melanoma by virtue of binding to melanin and that catechol-O-methyltransferase (COMT) may play a regulatory role in pigmentation.