Culture of mouse neural stem cell precursors.
Culture of mouse neural stem cell precursors.
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DOI：
10.3791/152
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发表时间：
2007-02-25
期刊：
影响因子：
--
通讯作者：
Monuki, Edwin S
中科院分区：
文献类型：
--
作者：
Currle, D Spencer;Hu, Jia Sheng;Monuki, Edwin S
原代神经干细胞培养对于研究中枢神经系统发育的机制是有用的。干细胞研究将增加我们对神经系统的了解，并可能使我们能够开发目前无法治愈的脑部疾病和损伤的治疗方法。此外，干细胞应用于干细胞研究，目的是详细研究神经分化和转分化的机制以及指导细胞特化为特定细胞类型的遗传和环境信号。这段视频演示了一种用于从胚胎第12.5天的小鼠背前脑中分离细胞的技术。解剖过程包括从子宫中收获E12.5小鼠胚胎，用精细的解剖钳去除“皮肤”，最后分离大脑皮层碎片。解剖后，组织被消化并机械分离。然后将重悬的解离细胞培养在有利于神经干细胞生长的“干细胞”培养基中。
Primary neural stem cell cultures are useful for studying the mechanisms underlying central nervous system development. Stem cell research will increase our understanding of the nervous system and may allow us to develop treatments for currently incurable brain diseases and injuries. In addition, stem cells should be used for stem cell research aimed at the detailed study of mechanisms of neural differentiation and transdifferentiation and the genetic and environmental signals that direct the specialization of the cells into particular cell types. This video demonstrates a technique used to disaggregate cells from the embryonic day 12.5 mouse dorsal forebrain. The dissection procedure includes harvesting E12.5 mouse embryos from the uterus, removing the "skin" with fine dissecting forceps and finally isolating pieces of cerebral cortex. Following the dissection, the tissue is digested and mechanically dissociated. The resuspended dissociated cells are then cultured in "stem cell" media that favors growth of neural stem cells.