SOMATIC HYBRID PLANTS FROM SEXUALLY INCOMPATIBLE WOODY SPECIES - CITRUS-RETICULATA AND CITROPSIS-GILLETIANA
SOMATIC HYBRID PLANTS FROM SEXUALLY INCOMPATIBLE WOODY SPECIES - CITRUS-RETICULATA AND CITROPSIS-GILLETIANA
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DOI：
10.1007/bf00269986
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发表时间：
1990-04-01
影响因子：
6.2
通讯作者：
CHANDLER, JL
中科院分区：
文献类型：
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作者：
GROSSER, JW;GMITTER, FG;CHANDLER, JL
异源四倍体柑橘属间体细胞杂种植株。克利奥帕特拉橘子和Citropsis gilletiana摇摆。和M·凯尔。(俗称吉利‘’S樱桃橙)经原生质体融合后再生。从胚珠来源的胚性悬浮培养中分离Cleopatra原生质体，并将其与Citropsis gilletiana的叶片来源的原生质体进行化学融合。利用体细胞胚胎发生技术，获得了克里奥帕特拉柑橘和体细胞杂种植株。根据叶片形态、根尖细胞染色体数目的差异以及磷酸葡萄糖变位酶(PGM)和磷酸己糖异构酶(PHI)同工酶带型的分析进行杂交植株鉴定。这是Rutaceae中报道的第一个不以中华柑橘(甜橙)为亲本的体细胞杂种，也是第一个由具有重要商业价值的柑橘砧木和互补但性别不相容的近缘物种产生的体细胞杂种。
Allotetraploid intergeneric somatic hybrid plants between Citrus reticulata Blanco cv. Cleopatra mandarin and Citropsis gilletiana Swing. and M. Kell. (common name Gillet''s cherry orange) were regenerated following protoplast fusion. Cleopatra protoplasts were isolated from an ovule-derived embryogenic suspension culture and fused chemically with leaf-derived protoplasts of Citropsis gilletiana. Cleopatra mandarin and somatic hybrid plants were regenerated via somatic embryogenesis. Hybrid plant identification was based on differential leaf morphology root-tip cell chromosome number, and electrophoretic analyses of phosphoglucose mutase (PGM) and phosphohexose isomerase (PHI) isozyme banding patterns. This is the first somatic hybrid within the Rutaceae reported that does not have Citrus sinensis (sweet orange) as a parent, and the first produced with a commercially important citrus rootstock and a complementry but sexually imcompatible, related species.