REGIONAL GAMMA-AMINOBUTYRIC ACID LEVELS IN RAT-BRAIN DETERMINED AFTER MICROWAVE FIXATION
REGIONAL GAMMA-AMINOBUTYRIC ACID LEVELS IN RAT-BRAIN DETERMINED AFTER MICROWAVE FIXATION
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DOI：
10.1111/j.1471-4159.1975.tb11650.x
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发表时间：
1975-01-01
影响因子：
4.7
通讯作者：
MEYERHOFF, JL
中科院分区：
文献类型：
--
作者：
BALCOM, GJ;LENOX, RH;MEYERHOFF, JL
—GABA levels in rat whole brain were compared following three methods of sacrifice: rapid microwave fixation, decapitation into liquid nitrogen, and decapitation at 20°C. Levels were shown to be identical in animals sacrificed by microwave fixation and decapitation into liquid nitrogen. In contrast, rats decapitated at 20°C had 18 per cent higher GABA levels when determined immediately post‐mortem and 48 per cent higher levels after 30 min at 20°C. Microwave treatment prevented these post‐mortem increases. The increase in GABA after decapitation at 20°C was even greater in hypothalamus than in whole brain.A comparison of 3 GABA extraction methods following microwave fixation demonstrated that sodium acetate was 88 per cent as effective as 80 per cent ethanol and more effective than 0·5n‐perchloric acid in extracting GABA.Fifteen brain regions were dissected from microwave‐treated brains and the GABA levels determined.
