Mesodermal cell fate decisions in Drosophila are under the control of the lineage genes numb, Notch, and sanpodo
Mesodermal cell fate decisions in Drosophila are under the control of the lineage genes numb, Notch, and sanpodo
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DOI：
10.1016/s0925-4773(98)00098-7
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发表时间：
1998-07-01
影响因子：
2.6
通讯作者：
Bodmer, R
中科院分区：
文献类型：
--
作者：
Park, M;Yaich, LE;Bodmer, R
在果蝇中，关于神经细胞命运的规范已经了解了很多，但对具有不同发育命运的中胚层细胞的谱系知之甚少。在发育初期，个别中胚层前体细胞被挑选出来，成为特定肌肉的创始细胞。肌肉创始细胞的选择被认为采用了Notch介导的侧向抑制过程，类似于观察到的神经前体的规范。然后，这些肌肉创始细胞似乎与周围未固定的肌细胞融合，诱导肌肉纤维合胞体的形成。相反，神经前体细胞的分化后代通常是定向的不对称细胞分裂的固定模式的结果。部分是由于Numb，一种定位于细胞内的受体蛋白，Sanpodo(Spdo)，一种潜在的原调节蛋白同系物，和Notch，一种跨膜受体蛋白的相互作用。在这里，我们研究了这些神经谱系基因在肌肉和心脏前体细胞命运指定中的作用。特别是，我们专注于一种祖细胞，它可能产生混合血统，既产生心包心脏细胞，又产生躯体肌肉创始细胞。我们发现，Numb不对称地分离到这些子细胞中的一个，通过阻止假定的肌肉创始人承担与其心脏兄弟姐妹相同的命运，对抗Notch和Spdo的功能。我们的结果表明，除了先前报道的诱导机制外，细胞不对称分裂在心脏和躯体肌肉模式中发挥着重要作用，此外，细胞骨架可能在细胞命运决定因素的不对称定位中发挥作用。(C)1998爱思唯尔爱尔兰科学有限公司。保留所有权利。
In Drosophila, much has been learned about the specification of neuronal cell fates but little is known about the lineage of mesodermal cells with different developmental fates. Initially in development, individual mesodermal precursor cells are singled out to become the founder cells for specific muscles. The selection of muscle founder cells is thought to employ a Notch-mediated process of lateral inhibition, similar to what is observed for the specification of neural precursors. These muscle founder cells then seem to fuse with the surrounding, uncommitted myocytes inducing the formation of muscle fiber syncytia. In contrast, the differentiated progeny of neural precursor cells are usually the result of a fixed pattern of asymmetric cell divisions which are directed. in part, by interactions between Numb, a localized intracellular-receptor protein, Sanpodo (Spdo), a potential tropomodulin homolog, and Notch, a transmembrane receptor protein. Here, we have investigated the role of these neural lineage genes in the cell fate specification of muscle and heart precursors. In particular, we have focused on a progenitor cell that is likely to produce a mixed lineage, generating both a pericardial heart cell and a somatic muscle founder cell. We show th;lt the asymmetric segregation of Numb into one of these daughter cells antagonizes the function of Notch and spdo by preventing the presumptive muscle founder from assuming the same fate as its cardiac sibling. Our results suggest that asymmetric cell divisions, in addition to the previously-documented inductive mechanisms, play a major role in cardiac and somatic muscle patterning and that additionally the cytoskeleton may have a role in the asymmetrical localization of cell fate determinants. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.