Expression profiling of the 14-3-3 gene family in response to salt stress and potassium and iron deficiencies in young tomato (Solanum lycopersicum) roots:: Analysis by real-time RT-PCR
Expression profiling of the 14-3-3 gene family in response to salt stress and potassium and iron deficiencies in young tomato (Solanum lycopersicum) roots:: Analysis by real-time RT-PCR
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DOI：
10.1093/aob/mcl189
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发表时间：
2006-11-01
期刊：
影响因子：
4.2
通讯作者：
Shi, Wei Ming
中科院分区：
文献类型：
--
作者：
Xu, Wei Feng;Shi, Wei Ming
背景和目的矿质营养缺乏和盐分是农业土壤上作物生长的主要限制因素。14-3-3蛋白是磷酸丝氨酸结合蛋白，通过直接的蛋白质-蛋白质相互作用调节多种靶标的活性，并且可能在响应矿物质营养素缺乏和盐胁迫中发挥重要作用。在本研究中，表达谱的14-3-3基因家族在盐胁迫和钾，铁缺乏年轻的番茄（Solanum lycopersicum）根进行了调查，以分析14-3-3蛋白在这些非生物stress.Methods序列的身份和系统发育树的创建进行DNAMAN版本4.0（Lynnon Biosoft公司）。Real-time RT-PCR检测了14-3-3基因在盐胁迫和钾、铁缺乏条件下的表达，系统发育树显示14-3-3基因家族福尔斯在番茄植株中分为两大类。通过使用实时RT-PCR，发现（a）在正常生长条件下，（B）14-3-3基因家族成员在番茄幼根中的mRNA水平存在显著差异，并且14-3-3蛋白在番茄根对盐胁迫和钾、铁缺乏的响应中表现出不同的基因表达模式。3蛋白质可能参与番茄幼根中盐胁迫和钾和铁缺乏信号传导途径，（B）番茄根中14-3-3基因家族成员的表达模式与相应蛋白质在系统发育树中的位置没有严格关系，（c）基因特异性表达模式表明番茄根的14-3-3基因家族中可能存在同工型特异性，（d）14-3-3蛋白（TFT 7）可能介导盐胁迫与番茄根缺钾和缺铁信号通路之间的相互作用。
Background and Aims Mineral nutrient deficiencies and salinity constitute major limitations for crop plant growth on agricultural soils. 14-3-3 proteins are phosphoserine-binding proteins that regulate the activities of a wide array of targets via direct protein-protein interactions and may play an important role in responses to mineral nutrients deficiencies and salt stress. In the present study, the expression profiling of the 14-3-3 gene family in response to salt stress and potassium and iron deficiencies in young tomato (Solanum lycopersicum) roots was investigated in order to analyse the 14-3-3 roles of the proteins in these abiotic stresses.Methods Sequence identities and phylogenetic tree creation were performed using DNAMAN version 4.0 (Lynnon Biosoft Company). Real-time RT-PCR was used to examine the expression of each 14-3-3 gene in response to salt stress and potassium and iron deficiencies in young tomato roots.Key Results The phylogenetic tree shows that the 14-3-3 gene family falls into two major groups in tomato plants. By using real-time RT-PCR, it was found that (a) under normal growth conditions, there were significant differences in the mRNA levels of 14-3-3 gene family members in young tomato roots and (b) 14-3-3 proteins exhibited diverse patterns of gene expression in response to salt stress and potassium and iron deficiencies in tomato roots.Conclusions The results suggest that (a) 14-3-3 proteins may be involved in the salt stress and potassium and iron deficiency signalling pathways in young tomato roots, (b) the expression pattern of 14-3-3 gene family members in tomato roots is not strictly related to the position of the corresponding proteins within a phylogenetic tree, (c) gene-specific expression patterns indicate that isoform-specificity may exist in the 14-3-3 gene family of tomato roots, and (d) 14-3-3 proteins (TFT7) might mediate cross-talk between the salt stress and potassium and iron-deficiency signalling pathways in tomato roots.