Regulation of the transcript for a lysosomal protein: evidence for a gene program modified by platelet-derived growth factor.
Regulation of the transcript for a lysosomal protein: evidence for a gene program modified by platelet-derived growth factor.
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溶酶体蛋白转录的调节：血小板衍生生长因子修饰基因程序的证据。
DOI：
10.1128/mcb.5.10.2582-2589.1985
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发表时间：
1985
影响因子：
5.3
通讯作者：
Scher,CD
中科院分区：
文献类型：
--
作者：
Frick,KK;Doherty,PJ;Gottesman,MM;Scher,CD
血小板衍生生长因子（PDGF）刺激密度阻滞的BALB/c-3T3细胞合成MEP，一种溶酶体蛋白。这种增强的合成似乎在很大程度上受到pdgf调节的MEP mRNA积累的调节，MEP mRNA是一个1.8千碱基的物种。MEP转录物的增加依赖于PDGF浓度，在添加PDGF后3至4小时开始，并在12小时达到最大。MEP转录物的积累是生长因子特异性的：PDGF和肿瘤启动子12- o - tetradecanoylphorbol13 -acetate（一种与PDGF作用类似的物质）诱导MEP RNA积累，而表皮生长因子、生长素C、胰岛素和全血浆则不会。自发转化的BALB/c-3T3细胞系（ST2-3T3）生长不需要PDGF，在缺乏PDGF的情况下，MEP RNA的表达最佳。pdgf调节的MEP RNA的增加不同于pdgf调节的c-myc和c-fosRNA的积累，因为它被环己亚胺阻断，这表明需要从头合成蛋白质。似乎PDGF通过一些转录本的积累来调节基因表达程序，以MEP为典型，依赖于其他转录本的翻译。
Platelet-derived growth factor (PDGF) stimulates density-arrested BALB/c-3T3 cells to synthesize MEP, a lysosomal protein. This enhanced synthesis appears to be largely regulated by the PDGF-modulated accumulation of MEP mRNA, a 1.8-kilobase species. The increase in the MEP transcript, which is dependent on the PDGF concentration, begins 3 to 4 h after PDGF addition and is maximal at 12 h. The accumulation of the MEP transcript is growth-factor specific: PDGF and the tumor promoter 12-O-tetradecanoylphorbol-13-acetate, an agent which acts like PDGF, induce MEP RNA accumulation, whereas epidermal growth factor, somatomedin C, insulin, and whole plasma do not. A spontaneously transformed BALB/c-3T3 cell line (ST2-3T3), which does not require PDGF for growth, optimally expresses MEP RNA in the absence of PDGF. The PDGF-modulated increase in MEP RNA is unlike PDGF-modulated c-mycand c-fosRNA accumulation because it is blocked by cycloheximide, suggesting a requirement for de novo protein synthesis. It appears that PDGF modulates a program of gene expression with the accumulation of some transcripts, typified by MEP, being dependent upon the translation of others.