A novel rice C2H2-type zinc finger protein lacking DLN-box/EAR-motif plays a role in salt tolerance
A novel rice C2H2-type zinc finger protein lacking DLN-box/EAR-motif plays a role in salt tolerance
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缺乏DLN-box/EAR-motif的新型水稻C2H2型锌指蛋白在耐盐性中发挥作用
DOI：
10.1016/j.bbaexp.2007.02.006
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发表时间：
2007-04-01
期刊：
影响因子：
--
通讯作者：
Zhang, Hong-Sheng
中科院分区：
文献类型：
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作者：
Huang, Ji;Yang, Xia;Zhang, Hong-Sheng
利用RT-PCR技术从水稻中克隆了一个编码C2 H2型锌指蛋白的基因ZFP 182的cDNA。ZFP 182编码一个分子量为18.2 kDa的蛋白质，具有两个C2 H2型锌指结构域、一个核定位信号和一个Leu丰富结构域。DLN-box/EAR-motif存在于大多数植物C2 H2型锌指蛋白中，但在ZFP 182中不存在。表达分析表明，ZFP 182基因在水稻成株期的叶、茎、根和穗中组成型表达，在幼苗期受低温（4 ℃）、150 mM NaCl和0.1 mM阿坝处理的诱导表达显著。将ZFP 182基因约1.4kb的启动子区与GUS报告基因融合，转化烟草。组织化学分析表明，GUS基因在正常条件下转化的烟草幼苗中未检测到表达，但在NaCl或KCl处理的烟草叶片和根的维管组织中有较强的表达。ZFP 182在转基因烟草中的表达和在水稻中的过表达提高了植物对盐胁迫的耐受性。这些结果表明，ZFP 182可能参与植物对盐胁迫的响应。(C)2007 Elsevier B. V.保留所有权利。
A cDNA for the gene ZFP182, encoding a C2H2-type zinc finger protein, was cloned from rice by RT-PCR. ZFP182 codes an 18.2 kDa protein with two C2H2-type zinc finger motifs, one nuclear localization signal and one Leu-rich domain. The DLN-box/EAR-motif, which exists in most of plant C2H2-type zinc finger proteins, does not exist in ZFP 182. The expression analysis showed that ZFP182 gene was constitutively expressed in leaves, culms, roots and spikes at the adult rice plants, and markedly induced in the seedlings by cold (4 degrees C), 150 mM NaCl and 0.1 mM ABA treatments. The approximate 1.4 kb promoter region of ZFP182 gene was fused into GUS reporter gene and transformed into tobacco. The histochemical analysis revealed that GUS expression could not be detected in transformed tobacco seedlings under normal conditions, but strongly observed in tobacco leaf discs and the vascular tissue of roots treated with NaCl or KCl. Expression of ZFP182 in transgenic tobacco and overexpression in rice increased plant tolerance to salt stress. These results demonstrated that ZFP 182 might be involved in plant responses to salt stress. (C) 2007 Elsevier B.V. All rights reserved.