Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell
Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell
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DEC1 过表达通过调节牛卫星细胞中的 MyoG 活性抑制肌原性分化
DOI：
10.1002/jcp.26471
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发表时间：
2018-12-01
影响因子：
5.6
通讯作者：
Chen, Hong
中科院分区：
文献类型：
--
作者：
Huang, Yongzhen;Lai, Xinsheng;Chen, Hong
分化胚胎软骨细胞1（DEC1）是碱性螺旋 - 环 - 螺旋转录因子Bhlhe40家族的成员，也被称为维甲酸刺激基因13（STRA13），在脂肪生成、肿瘤发生、外周生物钟输出、缺氧反应以及代谢综合征的发展调控中发挥着重要作用。先前的研究表明，DEC1参与骨骼肌发育；然而，其在成肌细胞分化中的具体作用尚未明确。在本研究中，我们发现DEC1在不同牛组织中广泛表达，且在分化的牛卫星细胞中表达下调。当通过CoCl₂模拟缺氧以及腺病毒介导的转导在牛卫星细胞中过表达DEC1时，肌肉特异性转录因子（Myf5、MyoD、MyoG和MHC）的表达显著下调。与此一致的是，通过荧光素酶报告基因分析进行的启动子分析也显示，牛DEC1的过表达能够抑制MyoG启动子活性。总之，DEC1的过表达通过抑制牛的MyoG启动子活性来阻断肌生成。我们的研究结果为肌肉生长提供了一种新机制，这将有助于提高牛肉产量。
Differentiated embryo chondrocyte 1 (DEC1), a member of basic-helix-loop-helix transcription factor Bhlhe40, also called stimulated by retinoic acid 13, STRA13, plays an important role in the regulation of adipogenesis, tumorigenesis, peripheral circadian output, response to hypoxia, and development of metabolic syndrome. Previous studies suggested that DEC1 was involved in skeletal muscle development; however, its precise role in myoblast differentiation has not been determined. In the present study, we showed that DEC1 expressed ubiquitously in different bovine tissues and was down-regulated in differentiated bovine satellite cells. Expression of muscle specific transcription factors (Myf5, MyoD, MyoG, and MHC) was significantly down-regulated when DEC1 was over-expressed by both CoCl2-simulated hypoxia and Adenovirus-mediated transduction in bovine satellite cells. Consistent with that, promoter analyses via luciferase reporter assay also revealed that overexpression of bovine DEC1 could inhibit MyoG promoter activity. In conclusion, overexpression of DEC1 blocked myogenesis by inhibiting MyoG promoter activity in bovine. Our results provided a new mechanism for the muscle growth, which would contribute to increase cattle meat productivity.