Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot
Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot
复制标题
法洛四联症婴儿DNMT1、DNMT3A、DNMT3B、MBD2 mRNA水平与LINE-1甲基化状态的相关性
DOI：
10.3892/ijmm.2013.1427
复制
发表时间：
2013-09-01
影响因子：
5.4
通讯作者：
Huang, Guoying
中科院分区：
文献类型：
--
作者：
Sheng, Wei;Qian, Yanyan;Huang, Guoying
DNA甲基化由DNA甲基转移酶（DNMT：DNMT 1、DNMT 3A和DNMT 3B）和甲基-CpG结合结构域蛋白2（MBD 2）催化和维持。然而，法乐四联症（tetralogy of Fallot，TOF）患者中DNMTs和MBD 2表达变化的生物学和临床意义及其与长散布核元件-1（long interspersed nuclear element-1，LINE-1）甲基化水平的关系尚不清楚。本研究采用定量RT-PCR（qRT-PCR）技术分析了DNMTs和MBD 2的mRNA水平。使用sequenom MassARRAY平台测量LINE-1的甲基化状态。TOF组DNMTs和MBD 2 mRNA水平显著低于对照组（P
DNA methylation is catalyzed and maintained by DNA methyltransferases (DNMTs: DNMT1, DNMT3A and DNMT3B) and methyl-CpG-binding domain protein 2 (MBD2). However, little is known about the biological and clinical significance of the expression changes of DNMTs and MBD2 and their association with the methylation levels of long interspersed nuclear element-1 (LINE-1) in patients with tetralogy of Fallot (TOF). In this study, quantitative RT-PCR (qRT-PCR) was applied to analyze the mRNA levels of DNMTs and MBD2. The methylation status of LINE-1 was measured using the sequenom MassARRAY platform. The mRNA levels of the DNMTs and MBD2 showed a statistically significant decrease in the patients with TOF (P