Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessment
Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessment
复制标题
DOI：
10.1093/humrep/deg199
复制
发表时间：
2003-04-01
影响因子：
6.1
通讯作者：
Kvist, U
中科院分区：
文献类型：
--
作者：
Björndahl, L;Söderlund, I;Kvist, U
背景：用于评估精子活力的一步曙红-黑色素染色技术是在20世纪50年代为各种哺乳动物开发的。虽然通常用于精液中的人类精子，但以前尚未发表过这种用途的验证。方法：对1235份连续精液样本进行了评价。研究结果：根据世界卫生组织标准（1992，1999）获得的精子活力数据进行评价时，一步曙红-黑色素染色技术给出了有效的结果。使用一步曙红-黑色素技术，染色（即假定死亡）和活动精子总和的平均值为91%（SD +/- 10%）。染色精子百分比和活动精子百分比的总和分布相似，无论样本中是否有大量或少量死亡精子。结论：基本精液分析的标准化和质量控制需要强大，可靠和简单的技术，易于学习，易于以相同的方式继续执行。一步曙红-黑色素技术不需要负相衬光学，但可以与普通的明场显微镜运行。由于它也包括较少的方法步骤来控制，它似乎更可取的标准化和质量控制管理方面。因此，在评估精子活力时，应推荐进行基本精液分析。
BACKGROUND: The one-step eosin-nigrosin staining technique for assessment of sperm vitality was developed in the 1950s for various mammalian species. Although commonly used on human sperm in semen, a validation for this use has not previously been published. METHODS: The technique was evaluated on 1235 consecutive semen samples. RESULTS: The one-step eosin-nigrosin staining technique gave valid results when evaluated with sperm motility data obtained according to World Health Organization standard (1992, 1999). The mean for the sums of stained (i.e. supposedly dead) and motile sperm using the one-step eosin-nigrosin technique was 91% (SD +/- 10%). The distribution of sums for percentage stained and percentage motile sperm was similar, regardless of whether the samples had many or few dead sperm. CONCLUSIONS: Standardization and quality control of basic semen analysis demands robust, reliable and simple techniques that are easy to learn, and easy to continue to perform in the same way. The one-step eosin-nigrosin technique does not need negative phase contrast optics but can be run with ordinary bright-field microscopy. Since it also includes fewer methodological steps to control, it seems preferable in terms of standardization and quality control management. It should therefore be recommended in the basic semen analysis when sperm vitality is to be assessed.