Responses of Well-differentiated Human Sinonasal Epithelial Cells to Allergen Exposure and Environmental Pollution in Chronic Rhinosinusitis
Responses of Well-differentiated Human Sinonasal Epithelial Cells to Allergen Exposure and Environmental Pollution in Chronic Rhinosinusitis
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DOI：
10.1177/1945892419853103
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发表时间：
2019-11-01
影响因子：
2.6
通讯作者：
Harvey, Richard J.
中科院分区：
文献类型：
--
作者：
Ahmadzada, Sejad;Ende, Jesse A.;Harvey, Richard J.
背景证据表明，内在细胞功能障碍导致慢性鼻窦炎 (CRS) 对环境触发因素的免疫反应失调。虽然流行病学和体内研究支持这一理论，但缺乏体外研究。方法使用气液界面培养模型培养来自人鼻窦粘膜的上皮细胞，产生分化良好的表型。标本被表征为慢性鼻窦炎伴（CRSwNP）或不伴（CRSsNP）鼻息肉和健康对照粘膜。将培养孔暴露于屋尘螨 (HDM)、柴油机尾气颗粒 (DPM) 或组合 (HDM+DPM) 24 小时以上，并比较 3 组的反应。测量纤毛跳动频率（CBF）和跨上皮电阻（TEER）以分别评估粘膜纤毛和屏障功能。 24 小时后测量白细胞介素 6 (IL-6) 和 33 (IL-33)。挑战测试后的结果表示为相对于基线的倍数变化。结果与对照相比，CRSsNP 中的基线 CBF 较低（5.270.51 Hz 与 5.88 +/- 1.22 Hz，P=.003）。与媒介物相比，HDM 显着降低了 CRSwNP 组的 CBF 和 TEER（CBF：0.55 +/- 0.25 vs 1.03 +/- 0.22，P
Background Evidence suggests that intrinsic cell dysfunction leads to dysregulated immune responses to environmental triggers in chronic rhinosinusitis (CRS). Although epidemiological and in vivo studies support this theory, in vitro studies are lacking.Methods Epithelial cells from human sinonasal mucosa were cultured using an air-liquid interface culture model producing a well-differentiated phenotype. Specimens were characterized as chronic rhinosinusitis with (CRSwNP) or without (CRSsNP) nasal polyps and healthy control mucosa. Culture wells were exposed to house dust mite (HDM), diesel exhaust particles (DPM), or a combination (HDM+DPM) over 24 hours and responses in the 3 groups compared. Ciliary beat frequency (CBF) and transepithelial electrical resistance (TEER) were measured to assess mucociliary and barrier function, respectively. Interleukin-6 (IL-6) and 33 (IL-33) were measured after 24 hours. Results following challenge testing are expressed as fold change from baseline.Results Baseline CBF was lower in CRSsNP compared with control (5.270.51 Hz vs 5.88 +/- 1.22 Hz, P=.003). HDM significantly reduced CBF and TEER in the CRSwNP group compared with its vehicle (CBF: 0.55 +/- 0.25 vs 1.03 +/- 0.22, P