Endocytosis in erythrocytes and their ghosts.
Endocytosis in erythrocytes and their ghosts.
复制标题
红细胞及其血影的内吞作用。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
K. Bensch
中科院分区：
文献类型：
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作者：
S. Schrier;B. Hardy;K. Bensch
对人红细胞及其幽灵的内吞作用的研究为阐明膜内陷和融合的步骤提供了机会。正在研究的特定制剂应该非常小心地描述，因为在几种可用的制剂中产生内吞作用的情况可能不同。例如，在重新密封的红鬼中，添加0.5到1 mM的钙可以刺激镁-三磷酸腺苷诱导的内吞作用，而在完整的红细胞中，添加钙可以刺激伯氨喹诱导的内吞作用，并抑制长春花碱诱导的内吞作用。此外，钙还能抑制白鬼的内吞作用。EDTA可以在白色幽灵中产生内吞作用，但在重新密封的红色幽灵中不能。在白色幽灵的研究中，与EDTA、胰酶或镁-三磷酸腺苷孵育可产生内吞作用，而预先加入抗幽灵蛋白抗体则可阻止内吞作用。当白色幽灵液泡被收获时，发现它们的幽灵蛋白已经耗尽。这些观察结果导致了一种假设，即内吞空泡是在细胞膜上基本上没有鬼影蛋白的区域形成的。
Study of endocytosis in human erythrocytes and their ghosts provides an opportunity for the elucidation of the steps involved in membrane invagination and fusion. The specific preparation being studied should be described with great care since circumstances that produce endocytosis may differ among the several available preparations. For example, in resealed red ghosts the addition of 0.5 to 1 mM Ca stimulates Mg-ATP-induced endocytosis, whereas in intact erythrocytes Ca addition stimulates primaquine-induced endocytosis and inhibits vinblastine-induced endocytosis. Furthermore, Ca inhibits endocytosis in white ghosts. EDTA can produce endocytosis in white ghosts but not in resealed red ghosts. In studies with white ghosts, incubation with EDTA, trypsin, or Mg-ATP produced endocytosis, whereas the prior addition of an antispectrin antibody prevented endocytosis. When the white ghost vacuoles were harvested they were found to be depleted of spectrin. These observations lead to the hypothesis that endocytic vacuoles are formed in areas of the membrane that have been substantially freed of spectrin.