Acid-Sensing Ion Channels Contribute to the Effect of Acidosis on the Function of Dendritic Cells
Acid-Sensing Ion Channels Contribute to the Effect of Acidosis on the Function of Dendritic Cells
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酸敏感离子通道有助于酸中毒对树突状细胞功能的影响
DOI：
10.4049/jimmunol.1001346
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发表时间：
2011-03-15
影响因子：
4.4
通讯作者：
Gong, Feili
中科院分区：
文献类型：
--
作者：
Tong, Jing;Wu, Wen-Ning;Gong, Feili
酸敏感离子通道（acid-sensing ion channels，ASICs）是变性蛋白/上皮Na+通道家族中的一个H+门控亚类，参与神经元的多种生理和病理过程。然而，关于ASIC在树突状细胞（DC）功能中的作用知之甚少。在这项研究中，我们研究了小鼠骨髓来源的DC中ASIC的表达及其在DC功能中的可能作用。我们发现ASIC 1、ASIC 2和ASIC 3在mRNA和蛋白水平上在DC中表达，并且细胞外酸可以在DC中诱发ASIC样电流。我们还证明，酸中毒上调CD 11 c，MHC II类，CD 80和CD 86的表达，并通过ASIC增强DC的Ag提呈能力。非甾体类抗炎药布洛芬和双氯芬酸可消除酸中毒对DCs的影响。这些结果表明，ASIC参与了酸中毒介导的对DC功能的影响。免疫学杂志，2011，186：3686-3692。
As an H+-gated subgroup of the degenerin/epithelial Na+ channel family, acid-sensing ion channels (ASICs) were reported to be involved in various physiological and pathological processes in neurons. However, little is known about the role of ASICs in the function of dendritic cells (DCs). In this study, we investigated the expression of ASICs in mouse bone marrow-derived DCs and their possible role in the function of DCs. We found that ASIC1, ASIC2, and ASIC3 are expressed in DCs at the mRNA and protein levels, and extracellular acid can evoke ASIC-like currents in DCs. We also demonstrated that acidosis upregulated the expression of CD11c, MHC class II, CD80, and CD86 and enhanced the Ag-presenting ability of DCs via ASICs. Moreover, the effect of acidosis on DCs can be abolished by the nonsteroidal anti-inflammatory drugs ibuprofen and diclofenac. These results suggest that ASICs are involved in the acidosis-mediated effect on DC function. The Journal of Immunology, 2011, 186: 3686-3692.