Bifidobacterium breve enhances transforming growth factor β1 signaling by regulating Smad7 expression in preterm infants
Bifidobacterium breve enhances transforming growth factor β1 signaling by regulating Smad7 expression in preterm infants
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DOI：
10.1097/01.mpg.0000228100.04702.f8
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发表时间：
2006-07-01
影响因子：
2.9
通讯作者：
Yamashiro, Yuichiro
中科院分区：
文献类型：
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作者：
Fujii, Tohru;Ohtsuka, Yoshikazu;Yamashiro, Yuichiro
目的：转化生长因子 (TGF) beta 1 在粘膜调节中表现出广泛的活性，包括诱导口服耐受、有效的抗炎作用、粘膜 IgA 表达以及对上皮细胞增殖和分化的影响。本研究探讨了益生菌对早产儿免疫系统与 TGF-β 信号传导的影响。方法：受试者包括 19 名早产儿，分为 2 组：接受短双歧杆菌补充剂（短双歧杆菌组）和未补充剂（对照组）。两组在出生后第0、14和28天采集血样。采用酶联免疫吸附法测定血清细胞因子水平，并采用半定量逆转录酶-聚合酶链式反应检测TGF-β信号分子Smad的表达水平。结果：短双歧杆菌组血清TGF-β1水平在第14天升高，并在第28天保持升高状态。相对于第 28 天的对照水平，施用短双歧杆菌后，Smad3 的信使 RNA 表达水平增强，而 Smad7（拮抗性 Smad）的信使 RNA 表达水平降低。 结论：这些结果表明，给早产儿施用短双歧杆菌可以上调 TGF-β 1 信号传导，并可能有利于减轻这些婴儿的炎症和过敏反应。
Objectives: Transforming growth factor (TGF) beta 1 displays a broad spectrum of activities in mucosal regulation, including induction of oral tolerance, potent anti-inflammatory effects, mucosal IgA expression and effects on epithelial cell proliferation and differentiation. The present study examined the effect of probiotics on the immunologic system of preterm infants in relation to TGF-beta signaling.Methods: Subjects comprised 19 preterm infants divided into 2 groups: receiving Bifidobacterium breve supplementation (B. breve group) and without supplementation (controls). Blood samples were collected from both groups on days 0, 14 and 28 after birth. Serum cytokine levels were measured using enzyme-linked immunosorbent assay, and expression levels of the TGF-beta signaling molecule, Smad, were examined using semiquantitative reverse transcriptase-polymerase chain reaction.Results: Serum TGF-beta 1 level was elevated on day 14 and remained elevated on day 28 in the B. breve group. Level of messenger RNA expression was enhanced for Smad3 and reduced for Smad7 (antagonistic Smad) after B. breve administration relative to levels in controls on day 28.Conclusions: These results demonstrated that the administration of B. breve to preterm infants can up-regulate TGF-beta 1 signaling and may possibly be beneficial in attenuating inflammatory and allergic reactions in these infants.