Development of transgenic alfalfa plants containing the foot and mouth disease virus structural polyprotein gene P1 and its utilization as an experimental immunogen
Development of transgenic alfalfa plants containing the foot and mouth disease virus structural polyprotein gene P1 and its utilization as an experimental immunogen
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DOI：
10.1016/j.vaccine.2004.11.014
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发表时间：
2005-03-07
期刊：
影响因子：
5.5
通讯作者：
Borca, MV
中科院分区：
文献类型：
--
作者：
Santos, MJD;Carrillo, C;Borca, MV
使用转基因植物作为表达病毒和细菌抗原的载体已作为生产实验疫苗的替代方法进行了越来越多的测试。在这里，我们报道了含有编码口蹄疫病毒（FMDV）多蛋白P1和蛋白酶3C基因的转基因苜蓿植物的生产。使用小鼠实验模型测试表达产物的免疫原性。肠胃外免疫的小鼠产生了强烈的抗体反应，并且在受到剧毒病毒攻击时得到了完全的保护。该报告证明了使用转基因植物表达口蹄疫多蛋白P1和蛋白酶3C的可能性以及它们作为有效的实验免疫原的利用。 (c) 2004 Elsevier Ltd. 保留所有权利。
The use of transgenic plants as vectors for the expression of viral and bacterial antigens has been increasingly tested as an alternative methodology for the production of experimental vaccines. Here, we report the production of transgenic alfalfa plants containing the genes encoding the polyprotein P1 and the protease 3C of foot and mouth disease virus (FMDV). The immunogenicity of the expressed products was tested using a mouse experimental model. Parenterally immunized mice developed a strong antibody response and were completely protected when challenged with the virulent virus. This report demonstrates the possibility of using transgenic plants to express polyprotein P1 and the protease 3C of FMDV and their utilization as effective experimental immunogens. (c) 2004 Elsevier Ltd. All rights reserved.