CARBOXYFLUORESCEIN - A PROBE OF THE BLOOD-OCULAR BARRIERS WITH LOWER MEMBRANE-PERMEABILITY THAN FLUORESCEIN
CARBOXYFLUORESCEIN - A PROBE OF THE BLOOD-OCULAR BARRIERS WITH LOWER MEMBRANE-PERMEABILITY THAN FLUORESCEIN
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DOI：
10.1001/archopht.1982.01030030637022
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发表时间：
1982-01-01
影响因子：
--
通讯作者：
LI, W
中科院分区：
文献类型：
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作者：
GRIMES, PA;STONE, RA;LI, W
研究了血眼屏障对羧基荧光素的渗透性，羧基荧光素是一种光谱性质相似但比荧光素极性更大的染料。羧基荧光素的辛醇-缓冲液分配比，作为脂溶性的指示测量，为约。在pH值为6.40-8.03之间，比荧光素低1000倍。两种染料的分配比显示出明显的pH依赖性。在大鼠中静脉注射后，通过荧光显微镜评价眼内染料分布。羧基荧光素不穿透睫状体或虹膜上皮细胞，而荧光素显著染色这些细胞。荧光强度的定量测量表明，羧基荧光素不进入视网膜，即使当高剂量给药。荧光素可以在整个视网膜中检测到，荧光强度水平与静脉内给药剂量成比例。羧基荧光素穿透血眼屏障的相对能力不是由与血浆蛋白的更大结合引起的，因为游离羧基荧光素的血浆浓度大于荧光素。显然，羧基荧光素作为血眼屏障的探针具有潜在的实验和临床用途。由于它的低膜渗透性，它可能会产生一个更好的定义屏障异常的性质比现在可能与荧光素。
The permeability of the blood-ocular barriers to carboxyfluorescein, a dye similar in spectral properties but more polar than fluorescein, was examined. Octanol-buffer partition ratios of carboxyfluorescein, measured as an indication of lipid solubility, were .apprx. 1000 times lower than those of fluorescein at pH values between 6.40-8.03. The partition ratios of both dyes show pronounced pH dependence. Intraocular dye distribution was evaluated by fluorescence microscopy after i.v. injection in rats. Carboxyfluorescein does not penetrate ciliary or iris epithelial cells, whereas fluorescein prominently stains these cells. Quantitative measurement of fluorescence intensity demonstrates that carboxyfluorescein does not enter the retina even when high doses are administered. Fluorescein can be detected throughout the retina with fluorescence intensity levels proportional to the i.v. dose administered. The relative inability of carboxyfluorescein to penetrate the blood-ocular barriers is not caused by greater binding to plasma proteins, since the plasma concentration of free carboxyfluorescein is greater than that of fluorescein. Apparently, carboxyfluorescein has potential experimental and clinical use as probe of the blood-ocular barriers. Because of its low membrane permeability, it may yield a better definition of the nature of barrier abnormalities than is now possible with fluorescein.