LINC00662 promotes gastric cancer cell growth by modulating the Hippo-YAP1 pathway
LINC00662 promotes gastric cancer cell growth by modulating the Hippo-YAP1 pathway
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LINC00662 通过调节 Hippo-YAP1 通路促进胃癌细胞生长
DOI：
10.1016/j.bbrc.2018.09.191
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发表时间：
2018-11-02
影响因子：
3.1
通讯作者：
Deng, Jun
中科院分区：
文献类型：
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作者：
Liu, Zhen;Yao, Yangyang;Deng, Jun
长链非编码RNA（lncRNA）作为各种疾病（特别是癌症）进展的重要调节因子发挥作用。在本研究中，利用癌症基因组图谱（TCGA）的数据集和一系列的细胞实验和临床组织样本分析，我们发现LINC00662表达显着上调胃癌（GC）组织和细胞系。LINC00662的高表达与低表达的患者相比预测不良预后。LINC00662表达的敲低降低了GC细胞的增殖并增加了GC细胞的化学敏感性。此外，我们证明了LINC00662的敲低抑制了GC细胞中的Hippo-YAP 1信号通路。在机制上，LINC00662通过吸收miR-497 - 5p来调节YAP 1介导的GC细胞增殖。总体而言，我们的结果揭示了LINC00662-miR-497 - 5p YAP 1轴在GC细胞生长中的关键作用，为GC提供了新的靶点。(C)2018爱思唯尔公司All rights reserved.
Long non-coding RNAs (lncRNAs) function as vital regulators of the progression of various diseases, particularly cancers. In the present study, utilizing the Cancer Genome Atlas (TCGA) data set and a series of cell experiments and clinical tissue samples assays, we found that LINC00662 expression was significantly up-regulated in gastric cancer (GC) tissues and cell lines. High expression of LINC00662 predicted poor prognosis compared to in patients showing low expression. Knockdown of LINC00662 expression decreased GC cell proliferation and increased the chemo-sensitivity of GC cells. Further, we demonstrated that knockdown of LINC00662 suppressed the Hippo-YAP1 signaling pathway in GC cells. Mechanistically, LINC00662 regulated YAP1-mediated GC cell proliferation by sponging miR-497-5p. Overall, our results revealed a critical role for the LINC00662-miR-497-5p YAP1 axis in GC cell growth, providing a new target for GC. (C) 2018 Elsevier Inc. All rights reserved.