Altered mucin core peptide expression in acute and chronic cholecystitis.
Altered mucin core peptide expression in acute and chronic cholecystitis.
复制标题
急性和慢性胆囊炎中粘蛋白核心肽表达的改变。
DOI：
10.1023/a:1005573213100
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发表时间：
2000
影响因子：
3.1
通讯作者：
Cherwitz,DL
中科院分区：
文献类型：
--
作者：
Ho,SB;Shekels,LL;Toribara,NW;Gipson,IK;Kim,YS;Purdum3rd,PP;Cherwitz,DL
人粘蛋白基因包括膜结合粘蛋白（MUC 1、MUC 3、MUC 4）和分泌粘蛋白（MUC 2、MUC 5AC、MUC 5 B、MUC 6）。我们的目的是确定粘蛋白基因在人胆囊细胞系，正常胆囊从肝脏供体（N = 7）和手术标本轻度慢性胆囊炎（N = 29），慢性胆囊炎（N = 48），急性和慢性胆囊炎（N = 27）的表达。MUC 1 mRNA是普遍存在的，但只有罕见的MUC 1免疫反应性检测。MUC 3、MUC 5AC、MUC 5 B和MUC 6 mRNA存在于所有胆囊标本和检查的细胞系中。MUC 3、MUC 5AC、MUC 5 B和MUC 6免疫反应性在86-100%的正常胆囊中存在。MUC 5AC阳性率在急性胆囊炎中明显降低（P < 0.05），而MUC 2阳性率在正常胆囊中为53.8%（P < 0.05），表面上皮以MUC 3、MUC 5AC和MUC 5 B为主，而深层粘膜皱襞以MUC 5 B和MUC 6为主。胆囊上皮细胞显示出一种独特而多样的粘蛋白核心蛋白模式，其随着炎症程度的增加而改变。
Human mucin genes include membrane-bound mucins (MUC1, MUC3, MUC4) and secretory mucins (MUC2, MUC5AC, MUC5B, MUC6). Our aim was to determine mucin gene expression in human gallbladder cell lines, normal gallbladder from liver donors(N = 7)and surgical specimens with mild chronic cholecystitis(N = 29),chronic cholecystitis(N = 48),and acute and chronic cholecystitis(N = 27).MUC1 mRNA was ubiquitous; however, only rare MUC1 immunoreactivity was detected. MUC3, MUC5AC, MUC5B, and MUC6 mRNA were present in all gallbladder specimens and cell lines examined. Prominent MUC3, MUC5AC, MUC5B, and MUC6 immunoreactivity was present in 86–100% of normal gallbladders. The frequency of MUC5AC reactivity was decreased in specimens with acute cholecystitis(P < 0.05).In contrast, MUC2-reactivity was absent in normal gallbladder and present in 53.8% of acute cholecystitis specimens(P < 0.05).Surface epithelium is characterized by MUC3, MUC5AC, and MUC5B, whereas deeper mucosal folds display MUC5B and MUC6 immunoreactivity. Gallbladder epithelium demonstrates a unique and diverse pattern of mucin core proteins that becomes altered with increasing degrees of inflammation.