Isolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochips.
Isolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochips.
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DOI：
10.1039/c3lc50580d
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发表时间：
2014-01-07
期刊：
影响因子：
6.1
通讯作者：
Wang SX
中科院分区：
文献类型：
--
作者：
Earhart CM;Hughes CE;Gaster RS;Ooi CC;Wilson RJ;Zhou LY;Humke EW;Xu L;Wong DJ;Willingham SB;Schwartz EJ;Weissman IL;Jeffrey SS;Neal JW;Rohatgi R;Wakelee HA;Wang SX
循环肿瘤细胞（CTC）的检测和表征可能揭示恶性疾病的诊断和治疗的见解。迄今为止开发的分离 CTC 的技术受到一种或多种限制，例如通量低、无法释放捕获的细胞以及依赖昂贵的仪器进行富集或后续表征。我们报告了磁分离装置磁筛的持续开发，它是一种具有密集磁孔阵列的微型微流控芯片。它可以从全血中高效捕获用磁性纳米粒子标记的肿瘤细胞，并具有高通量和捕获细胞的高效释放。为了后续对 CTC 进行表征，我们使用带有巨磁阻纳米传感器的蛋白质芯片进行了测定，对用磁性筛子富集的 CTC 进行了突变分析。这些磁性技术是独立的设备，它们的使用可能会为癌症患者的“液体活检”的常规制备和表征开辟道路。
Detection and characterization of circulating tumor cells (CTCs) may reveal insights into the diagnosis and treatment of malignant disease. Technologies for isolating CTCs developed thus far suffer from one or more limitations, such as low throughput, inability to release captured cells, and reliance on expensive instrumentation for enrichment or subsequent characterization. We report a continuing development of a magnetic separation device, the magnetic sifter, which is a miniature microfluidic chip with a dense array of magnetic pores. It offers high efficiency capture of tumor cells, labeled with magnetic nanoparticles, from whole blood with high throughput and efficient release of captured cells. For subsequent characterization of CTCs, an assay, using a protein chip with giant magnetoresistive nanosensors, has been implemented for mutational analysis of CTCs enriched with the magnetic sifter. The use of these magnetic technologies, which are separate devices, may lead the way to routine preparation and characterization of “liquid biopsies” from cancer patients.
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影响因子：
45.3
作者：
Cristofanilli, M;Hayes, DF;Terstappen, LWMM
通讯作者：
Terstappen, LWMM
影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
DOI：
10.1186/bcr2131
发表时间：
2008
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Deng G;Herrler M;Burgess D;Manna E;Krag D;Burke JF
通讯作者：
Burke JF
影响因子：
17.1
作者：
Stott SL;Lee RJ;Nagrath S;Yu M;Miyamoto DT;Ulkus L;Inserra EJ;Ulman M;Springer S;Nakamura Z;Moore AL;Tsukrov DI;Kempner ME;Dahl DM;Wu CL;Iafrate AJ;Smith MR;Tompkins RG;Sequist LV;Toner M;Haber DA;Maheswaran S
通讯作者：
Maheswaran S
影响因子：
6.1
作者：
Kang, Joo H.;Krause, Silva;Ingber, Donald E.
通讯作者：
Ingber, Donald E.