Dynamic Subcellular Localization, Accumulation, and Interactions of Proteins FromTomato Yellow Leaf Curl China Virusand Its Associated Betasatellite
Dynamic Subcellular Localization, Accumulation, and Interactions of Proteins FromTomato Yellow Leaf Curl China Virusand Its Associated Betasatellite
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DOI：
10.3389/fpls.2020.00840
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发表时间：
2020-06-16
影响因子：
5.6
通讯作者：
Zhou, Xueping
中科院分区：
文献类型：
--
作者：
Li, Hao;Li, Fangfang;Zhou, Xueping
双生病毒属是植物病毒中种类最多的一种，在世界范围内引起毁灭性的作物病害。对这些病毒的耐药性的发展将需要对病毒蛋白质功能和相互作用有清楚的了解。中国番茄黄化曲叶病毒（Tomato yellow leaf curl China virus，TYLCCNV）是一种典型的单分型双生病毒，在田间与中国番茄黄化曲叶病毒（Tomato yellow leaf curl China betasatellite，TYLCCNV/TYLCCNB）共生，复合侵染给茄科植物造成严重的经济损失。由TYLCCNV/TYLCCNB复合物编码的每种蛋白质的功能尚未以靶向方式进行检查。在这里，我们展示了TYLCCNV编码的六种病毒蛋白和TYLCCNB编码的β C1蛋白随着时间的推移在植物中的动态亚细胞定位和积累，并分析了TYLCCNV或TYLCCNV/TYLCCNB感染对这些参数的影响。在这项研究中还测试了七种病毒蛋白之间的相互作用：C2在病毒蛋白相互作用网络中起着中心作用，因为它与C3，C4，V2和β C1相互作用。C1、C2和V2也存在自相互作用。总之，本文提供的数据提供了一个模板，用于研究病毒蛋白质随时间推移的功能，无论是否有病毒感染，并指出C2作为一个关键蛋白质可能在病毒生命周期的协调中发挥核心作用。
Geminiviruses contain the largest number of species of plant viruses, and cause devastating crop diseases worldwide. The development of resistance to these viruses will require a clear understanding of viral protein function and interactions. Tomato yellow leaf curl China virus (TYLCCNV) is a typical monopartite geminivirus, which is associated with a tomato yellow leaf curl China betasatellite (TYLCCNB) in the field; the complex infection of TYLCCNV/TYLCCNB leads to serious economic losses in solanaceous plants. The functions of each protein encoded by the TYLCCNV/TYLCCNB complex have not yet been examined in a targeted manner. Here, we show the dynamic subcellular localization and accumulation of six viral proteins encoded by TYLCCNV and the beta C1 protein encoded by TYLCCNB in plants over time, and analyzed the effect of TYLCCNV or TYLCCNV/TYLCCNB infection on these parameters. The interaction among the seven viral proteins was also tested in this study: C2 acts as a central player in the viral protein interaction network, since it interacts with C3, C4, V2, and beta C1. Self-interactions were also found for C1, C2, and V2. Together, the data presented here provide a template for investigating the function of viral proteins with or without viral infection over time, and points at C2 as a pivotal protein potentially playing a central role in the coordination of the viral life cycle.