Fabrication of a cell array on ultrathin hydrophilic polymer gels utilising electron beam irradiation and UV excimer laser ablation
Fabrication of a cell array on ultrathin hydrophilic polymer gels utilising electron beam irradiation and UV excimer laser ablation
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DOI：
10.1016/j.biomaterials.2005.01.021
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发表时间：
2005-09-01
期刊：
影响因子：
14
通讯作者：
Okano, T
中科院分区：
文献类型：
--
作者：
Iwanaga, S;Akiyama, Y;Okano, T
目前应用的大多数表面图案化方法是基于光刻技术和硅或玻璃基板上的微加工。在这里，我们报告了一种新的方法，通过利用电子束（EB）聚合和局部激光烧蚀技术在聚苯乙烯衬底上接枝超薄细胞排斥聚合物层来制备图案表面。用EB辐照将聚丙烯酰胺接枝到组织培养聚苯乙烯（TCPS）培养皿上。经ATR/FT-IR测定，这些PAAm接枝的TCPS表面的水接触角小于10度（cos θ = 0.99）， PAAm接枝量为1.6 μ g/cm(2)。紫外准分子激光（ArF: 193 nm）烧蚀成功制备了由亲水性PAAm和疏水性聚苯乙烯基层组成的微图纹表面。37℃下，15 μ g/mL纤维连接蛋白预处理后，牛颈动脉内皮细胞仅粘附在消融结构域上，消融结构域的大小显著影响占据每个结构域的细胞数量。在不损失模式保真度的情况下，图像化表面的细胞图像化功能维持了2个多月，这表明与之前报告中描述的自组装单层烷硫醇制备的细胞阵列相比，可以获得更持久的细胞阵列。这里提出的表面制造技术可以用于制备基于细胞的生物传感器以及组织工程结构。(c) 2005 Elsevier Ltd版权所有。
Most of the surface patterning methods currently applied are based on lithography techniques and microfabrication onto silicon or glass substrates. Here we report a novel method to prepare patterned surfaces on polystyrene substrates by grafting ultrathin cell-repellent polymer layers utilising both electron beam (EB) polymerisation and local laser ablation techniques for microfabrication. Polyacrylamide was grafted onto tissue culture polystyrene (TCPS) dishes using EB irradiation. Water contact angles for these PAAm-grafted TCPS surfaces were less than 10 degrees (cos theta = 0.99) with PAAm grafted amounts of 1.6 mu g/cm(2) as determined by ATR/FT-IR. UV excimer laser (ArF: 193 nm) ablation resulted in the successful fabrication of micropatterned surfaces composed of hydrophilic PAAm and hydrophobic basal polystyrene layers. Bovine carotid artery endothelial cells adhered only to the ablated domains after pretreatment of the patterned surfaces with 15 mu g/mL fibronectin at 37 degrees C. The ablated domain sizes significantly influenced the number of cells occupying each domain. Cell patterning functionality of the patterned surfaces was maintained for more than 2 months without loss of pattern fidelity, indicating that more durable cell arrays can be obtained compared to those prepared by self-assembled monolayers of alkanethiols, as described in previous reports. The surface fabrication techniques presented here can be utilised for the preparation of cell-based biosensors as well as tissue engineering constructs. (c) 2005 Elsevier Ltd. All rights reserved.