NERP-2 regulates gastric acid secretion and gastric emptying via the orexin pathway.
NERP-2 regulates gastric acid secretion and gastric emptying via the orexin pathway.
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NERP-2 通过食欲素途径调节胃酸分泌和胃排空。
DOI：
10.1016/j.bbrc.2017.02.064
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Masamitsu Nakazato.
中科院分区：
文献类型：
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作者：
Cherl Namkoong;Koji Toshinai;Zaved Waise;Hideyuki Sakoda;Kazuki Sasaki;Yoichi Ueta;Min-Seon Kim;Naoto Minamino;Masamitsu Nakazato.
神经内分泌调节肽（NERP）-2来源于VGF的一个不同区域，VGF是一种神经分泌蛋白，最初被鉴定为大鼠PC 12细胞中神经生长因子反应基因的产物。下丘脑外侧核中NERP-2与食欲素A的共定位增加了食欲素A诱导的大鼠和小鼠的摄食和能量消耗。下丘脑中的促食欲肽和厌食肽调节胃功能。本研究探讨了NERP-2对大鼠胃功能的影响。大鼠侧脑室注射NERP-2可增加胃酸分泌和胃排空，而外周注射不影响胃功能。食欲素1受体拮抗剂SB 334867可阻断NERP-2诱导的胃酸分泌和胃排空。NERP-2还诱导下丘脑外侧核和迷走X背运动核中的Fos表达，这是中枢神经系统中调节胃功能的关键部位。迷走神经传出信号传导阻断剂阿托品可完全阻断NERP-2诱导的胃酸分泌。这些结果表明，NERP-2的中枢给药激活食欲素途径，导致胃酸分泌和胃排空升高。
Neuroendocrine regulatory peptide (NERP)-2 is derived from a distinct region of VGF, a neurosecretory protein originally identified as a product of a nerve growth factor-responsive gene in rat PC12 cells. Colocalization of NERP-2 with orexin-A in the lateral hypothalamus increases orexin-A-induced feeding and energy expenditure in both rats and mice. Orexigenic and anorectic peptides in the hypothalamus modulate gastric function. In this study, we investigated the effect of NERP-2 on gastric function in rats. Intracerebroventricular administration of NERP-2 to rats increased gastric acid secretion and gastric emptying, whereas peripheral administration did not affect gastric function. NERP-2-induced gastric acid secretion and gastric emptying were blocked by an orexin 1 receptor antagonist, SB334867. NERP-2 also induced Fos expression in the lateral hypothalamus and the dorsomotor nucleus of the vagus X, which are key sites in the central nervous system for regulation of gastric function. Atropine, a blocker of vagal efferent signal transduction, completely blocked NERP-2-induced gastric acid secretion. These results demonstrate that central administration of NERP-2 activates the orexin pathway, resulting in elevated gastric acid secretion and gastric emptying.