Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains
Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains
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DOI：
10.1128/jcm.37.11.3556-3563.1999
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发表时间：
1999-11-01
影响因子：
9.4
通讯作者：
Kreiswirth, BN
中科院分区：
文献类型：
--
作者：
Shopsin, B;Gomez, M;Kreiswirth, BN
本文对320株金黄色葡萄球菌（Staphylococcus aureus）进行了A蛋白基因X区（spa）的DNA序列分析，并与表型和分子生物学方法进行了比较。将金黄色葡萄球菌菌株分成与流行病学信息相关的组。对两个已鉴定的研究群体进行了研究，收集了59个分离株（F，C。Tenover，R，Arbeit，G。Archer，J. Piddle，S，Byrne，R，Goering，G.汉考克，G. A. Hebert，B，Hill，R. Hollis，W. Jarvis，B，Kreismartth，W，Barner，J. Maslow，L，K，McDougal，J，M.米勒，M，Mulligan，和M. A. Pfaller，J. Clin，Microbiol. 32：407-415，1994）用于测试区分爆发与流行病学无关菌株的能力。一项多中心研究（R，B）单独收集了261株分离株。罗伯茨，A。de Lencastre，W，Reinner，E，P，Severina，B. Shopsin、B、N. Kreisperth和A. Tomasz，J. Infect. 178：164-171，1998）。在纽约市（NYC）的金黄色葡萄球菌中，将SPA分型将菌株沿着克隆系分组的能力与脉冲场凝胶电泳和Southern杂交的组合的能力进行比较。在研究的320株分离株中，spa分型鉴定出24种不同的重复类型和33种不同的菌株类型。SPA分型区分了29个相关菌株中的27个，并且没有为来自CDC收集的四次爆发的4个无关菌株提供独特的指纹。在NYC收集中，SPA分型提供了先前描述的五个主要组中195个菌株中的185个的克隆分配。spa测序是一种高效的快速分型方法。金黄色葡萄球菌，尽管特异性有一定的代价，但在速度、易用性、易于解释和实验室标准化方面具有显著的优势。
Three hundred and twenty isolates of Staphylococcus aureus were typed by DNA sequence analysis of the X region of the protein A gene (spa), spa typing was compared to both phenotypic and molecular techniques for the ability to differentiate and categorize S. aureus strains into groups that correlate with epidemiological information. Two previously characterized study populations mere examined, A collection of 59 isolates (F, C. Tenover, R, Arbeit, G. Archer, J. Piddle, S, Byrne, R, Goering, G. Hancock, G. A. Hebert, B, Hill, R. Hollis, W, R. Jarvis, B, Kreiswirth, W, Eisner, J. Maslow, L, K, McDougal, J, M. Miller, M, Mulligan, and M. A. Pfaller, J. Clin, Microbiol. 32:407-415, 1994) from the Centers fbr Disease Control and Prevention (CDC) was used to test for the ability to discriminate outbreak from epidemiologically unrelated strains. A separate collection of 261 isolates form a multicenter study (R, B. Roberts, A. de Lencastre, W, Eisner, E, P, Severina, B. Shopsin, B, N. Kreiswirth, and A. Tomasz, J. Infect. Dis. 178:164-171, 1998) of methicillin-resistant S. aureus in New York City (NYC) was used to compare the ability of spa typing to group strains along clonal lines to that of the combination of pulsed-field gel electrophoresis and Southern hybridization. In the 320 isolates studied, spa typing identified 24 distinct repeat types and 33 different strain types. spa typing distinguished 27 of 29 related strains and did not provide a unique fingerprint for 4 unrelated strains from the four outbreaks of the CDC collection. In the NYC collection, spa typing provided a clonal assignment for 185 of 195 strains within the five major groups previously described. spa sequencing appears to be a highly effective rapid typing tool for S. aureus that, despite some expense of specificity, has significant advantages in terms of speed, ease of use, ease of interpretation, and standardization among laboratories.