Peromyscus leucopus mice: a potential animal model for haematological studies.
Peromyscus leucopus mice: a potential animal model for haematological studies.
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Peromyscus leucopus 小鼠：血液学研究的潜在动物模型。
DOI：
10.1111/iep.12091
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发表时间：
2014
影响因子：
3
通讯作者：
Young,NealS
中科院分区：
文献类型：
--
作者：
Sun,Yu;Desierto,MarieJ;Ueda,Yasutaka;Kajigaya,Sachiko;Chen,Jichun;Young,NealS
白足鼠与实验室小鼠（MM）有着相似的身体特征，但具有更高的敏捷性和更长的寿命。本文比较了驯化的白腹鼠（PLL）和小家鼠（MMB 6）的外周血（PB）、骨髓（BM）和脾脏的细胞组成。PLL小鼠血液中血小板显著减少，单核细胞显著增加，BM中巨核细胞显著减少。PLL小鼠的脾脏明显较小，细胞减少50%，“红髓”减少。PLL小鼠在2-8月龄和16-26月龄之间没有明显的血液学变化，除了血液单核细胞显著增加。细胞活性氧（ROS）含量随年龄的增长无明显变化，但不同细胞类型之间差异显着。用饮用水中的抗氧化剂N-乙酰半胱氨酸治疗2至8个月大的PLL小鼠3个月，不影响细胞ROS含量，但增加了血液白细胞，特别是单核细胞的浓度。PLL小鼠中的低血小板、低巨核细胞、高单核细胞和低脾红细胞生成比在MMB 6中观察到的值更接近人类测量值。
Peromyscus leucopusmice share physical similarities with laboratory miceMus musculus(MM) but have higher agility and longer lifespan. We compared domesticatedP. leucopus linville(PLL) andM. musculusC57BL/6 (MMB6) mice for cellular composition of peripheral blood (PB), bone marrow (BM) and spleen. PLL mice had significantly fewer platelets and significantly more monocytes in the blood, and notably fewer megakaryocytes in the BM. Spleens of PLL mice were significantly smaller, with 50% fewer cells and reduced ‘red pulp’. There was no obvious haematological change in PLL mice between 2–8 and 16–26 months of age, except for a significant increase in blood monocytes. Cellular reactive oxygen species (ROS) content showed no change with age but differed significantly between different cell types. Treating two to eight month‐old PLL mice with antioxidant N‐acetylcysteine in drinking water for three months did not affect cellular ROS content, but increased blood leucocytes especially the concentration of monocytes. The low platelets, low megakaryocytes, high monocytes and low splenic erythropoiesis in PLL mice resemble human measurements better than the values seen in MMB6.