Analysis of the microcystin-LR production ability of metagenomic mcy genes in freshwater aquaculture ponds focusing on the abundance of metagenomic mcy genes and SNP
Analysis of the microcystin-LR production ability of metagenomic mcy genes in freshwater aquaculture ponds focusing on the abundance of metagenomic mcy genes and SNP
复制标题
淡水养殖池塘宏基因组mcy基因产微囊藻毒素-LR能力分析，重点关注宏基因组mcy基因丰度和SNP
DOI：
10.15666/aeer/1901_237248
复制
发表时间：
2020
影响因子：
0.7
通讯作者：
Dajuan Zhang
中科院分区：
文献类型：
--
作者：
Shaojie Dong;Xiangdong Bi;Junyi Zhang;Wei Dai;Peipei Zhang;Keru Zhou;Xueying Wang;Dajuan Zhang
MCs污染已成为淡水养殖的世界性问题，MC-LR由于其强烈的肝毒性而引起了人们的广泛关注。为分析野外蓝藻产MC-LR能力，于2018年7月在5个淡水鱼塘调查了蓝藻产MC-LR能力与MC-LR含量、TN含量、TP含量、宏基因组mcy基因SNP的关系。结果表明，在氮磷充足条件下，MC-LR含量与宏基因组mcy基因A-J丰度呈显著正相关，而宏基因组mcy基因A-J产生MC-LR的能力与宏基因组mcy基因丰度、MC-LR含量、TN和TP含量无显著相关性。18个SNPs与MC-LR的产生能力显著正相关，388个SNPs与MC-LR的产生能力显著负相关。铜绿微囊藻（Microcystis aeruginosa）NIES 843的mcy C和mcy B基因型最有利于MC-LR的产生。可能存在某种机制来避免mcy C基因突变引起的底物特异性改变。
MCs pollution have become a worldwide problem for freshwater aquaculture, and MC-LR has attracted considerable attention due to its potent hepatotoxicity. To analysis the MC-LR production ability of cyanobacteria in field, the relationship between the MC-LR production ability and MC-LR content, TN content, TP content, SNP of the metagenomic mcy genes were investigated in 5 freshwater fishponds during July 2018. The results showed that MC-LR content was significantly positively correlated with the abundance of metagenomic mcy gene A-J, while the MC-LR production ability of metagenomic mcy gene A-J was in indistinctive correlation with the abundance of metagenomic mcy gene, and MC-LR content, and the content of TN and TP in sufficient nitrogen and phosphate conditions. 18 SNPs significantly positively correlated with the MC-LR production ability and 388 SNPs significantly negatively correlated with the MC-LR production ability. The genotype of mcy C and mcy B in Microcystis aeruginosa NIES 843 was the most favorable for the MC-LR production in present study. There might be some mechanism to avoid substrate specific changes caused by genetic mutation of mcy C。