Quantitative Study of Cell Invasion Process under Extracellular Stimulation of Cytokine in a Microfluidic Device.
Quantitative Study of Cell Invasion Process under Extracellular Stimulation of Cytokine in a Microfluidic Device.
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DOI：
10.1038/srep25557
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发表时间：
2016-05-06
影响因子：
4.6
通讯作者：
Tsang NM
中科院分区：
文献类型：
--
作者：
Lei KF;Tseng HP;Lee CY;Tsang NM
细胞侵袭是癌症转移的第一步，癌症转移是癌症患者死亡的主要原因，并被定义为细胞通过细胞外基质（ECM）的运动。研究细胞侵袭性与细胞外刺激的关系对于抑制肿瘤的转移性播散至关重要。传统的细胞侵袭测定是基于Boyden室测定，其具有许多局限性。本研究开发了一种结合阻抗测量技术的微流控装置，用于定量研究细胞侵袭过程。该装置由2个与填充有水凝胶的微通道连接的储液器组成。恶性细胞沿着微通道侵入，并且通过测量位于微通道底部的电极同时进行阻抗测量。因此，可以实时和非侵入性的方式监测细胞的侵入过程。此外，然后计算细胞侵袭率以研究细胞侵袭和细胞外刺激之间的相关性，即，IL-6细胞因子。结果表明，细胞侵袭率与IL-6浓度成正比。微流控装置为基于细胞的测定提供了可靠和方便的平台，以促进癌症研究中的更多定量评估。
Cell invasion is the first step of cancer metastasis that is the primary cause of death for cancer patients and defined as cell movement through extracellular matrix (ECM). Investigation of the correlation between cell invasive and extracellular stimulation is critical for the inhabitation of metastatic dissemination. Conventional cell invasion assay is based on Boyden chamber assay, which has a number of limitations. In this work, a microfluidic device incorporating with impedance measurement technique was developed for quantitative investigation of cell invasion process. The device consisted of 2 reservoirs connecting with a microchannel filled with hydrogel. Malignant cells invaded along the microchannel and impedance measurement was concurrently conducted by measuring across electrodes located at the bottom of the microchannel. Therefore, cell invasion process could be monitored in real-time and non-invasive manner. Also, cell invasion rate was then calculated to study the correlation between cell invasion and extracellular stimulation, i.e., IL-6 cytokine. Results showed that cell invasion rate was directly proportional to the IL-6 concentration. The microfluidic device provides a reliable and convenient platform for cell-based assays to facilitate more quantitative assessments in cancer research.