CHARACTERIZATION OF X-CHROMOSOME SPECIFIC SATELLITE DNA OF MUNTIACUS-MUNTJAK-VAGINALIS
CHARACTERIZATION OF X-CHROMOSOME SPECIFIC SATELLITE DNA OF MUNTIACUS-MUNTJAK-VAGINALIS
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DOI：
10.1007/bf00333506
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发表时间：
1982-01-01
期刊：
影响因子：
1.6
通讯作者：
FITTLER, F
中科院分区：
文献类型：
--
作者：
BOGENBERGER, J;SCHNELL, H;FITTLER, F
用限制性内切酶和克隆DNA片段杂交实验，从阴道毛滴虫培养细胞中分离到一个高度重复的DNA(命名为卫星IA)，并对其结构进行了分析。几种限制性核酸酶将卫星IA DNA切割成一系列片段，这些片段是800bp[碱基对]基本重复单位的倍数。在阴道支原体(卫星IB)的第二个高度重复的DNA组分中也发现了与卫星IA DNA同源的序列。它的结构比卫星IA更复杂，不符合基本重复单元的简单周期性。原位杂交显示，这两个卫星都完全局限于X染色体，它们位于靠近着丝粒的两个臂上。在被认为与X染色体长臂同源的Y1染色体上未发现卫星DNA。这些结果对X染色体的进化有着有趣的启示。
A highly repeated DNA (designated satellite IA) was isolated from cultured cells of M. muntjak vaginalis and its organization analyzed by the use of restriction nucleases and hybridization experiments with cloned DNA-fragments. Several restriction nucleases cleave the satellite IA DNA into a series of fragments, which are multiples of a basic repeat unit of 800 bp [base pair]. Sequences homologous to the satellite IA DNA were aslo found in a 2nd highly repetitive DNA component of M. muntjak vaginalis (satellite IB). Its organization is more complex than the one of satellite IA and does not conform to a simple periodicity of a basic repeat unit. Hybridization in situ revealed, that both satellites are confined in their entirety to the X-chromosome, where they are located at both arms close to the centromere. No satellite DNA was found at the Y1-chromosome, which is considered to be homologous to the long arm of the X-chromosome. These results have interesting implications for the evolution of the X-chromosome.