A putative peroxisomal polyamine oxidase, AtPAO4, is involved in polyamine catabolism in Arabidopsis thaliana
A putative peroxisomal polyamine oxidase, AtPAO4, is involved in polyamine catabolism in Arabidopsis thaliana
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DOI：
10.1093/pcp/pcn114
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发表时间：
2008-09-01
影响因子：
4.9
通讯作者：
Nishimura, Mikio
中科院分区：
文献类型：
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作者：
Kamada-Nobusada, Tomoe;Hayashi, Makoto;Nishimura, Mikio
我们鉴定了三个拟南芥多胺氧化酶基因：AtPAO2、AtPAO3 和 AtPAO4。这些基因作为单体红色荧光蛋白融合蛋白在拟南芥根细胞中瞬时表达表明它们都是过氧化物酶体蛋白。对它们在不同器官中的转录本的定量分析表明，AtPAO4 是根过氧化物酶体中的主要亚型。重组AtPAO4蛋白的分析表明，它是一种黄素蛋白，催化精胺氧化转化为亚精胺。通过使用 T-DNA 插入和 RNA 干扰技术建立的 AtPAO4 缺陷突变体显着增加了根中的精胺水平并降低了亚精胺水平。这些结果表明AtPAO4是参与多胺分解代谢的根过氧化物酶体多胺氧化酶。微阵列分析表明，AtPAO4 缺乏会导致与干旱胁迫反应和类黄酮生物合成相关的基因表达发生变化。
We characterized three Arabidopsis polyamine oxidase genes, AtPAO2, AtPAO3 and AtPAO4. Transient expression of these genes as monomeric red fluorescent protein fusion proteins in Arabidopsis root cells revealed that all are peroxisomal proteins. Quantitative analysis of their transcripts in various organs suggested that AtPAO4 is the major isoform in root peroxisomes. Analysis of recombinant AtPAO4 protein indicated that it is a flavoprotein that catalyzed the oxidative conversion of spermine to spermidine. AtPAO4-deficient mutants established by using T-DNA insertion and RNA interference techniques had markedly increased spermine and decreased spermidine levels in the roots. These results suggest that AtPAO4 is a root peroxisomal polyamine oxidase that participates in polyamine catabolism. Microarray analysis showed that AtPAO4 deficiency induced alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis.