Temporal-Spatial Expressions of Spy1 in Rat Sciatic Nerve After Crush
Temporal-Spatial Expressions of Spy1 in Rat Sciatic Nerve After Crush
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DOI：
10.1007/s10571-012-9887-2
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发表时间：
2013-03-01
影响因子：
4
通讯作者：
Gu, Xingxing
中科院分区：
文献类型：
--
作者：
Cao, Jianhua;Yang, Jiao;Gu, Xingxing
Spy1是一种新的细胞周期蛋白，在紫外线照射下增强细胞增殖，促进G1/S转变，抑制细胞凋亡。在乳腺发育过程中，Spy1水平受到严格调控，体内过度表达Spy1会加速肿瘤的发生。但对Spy1在周围神经系统损伤和再生的病理过程中的作用知之甚少。本实验建立大鼠坐骨神经压迫（SNC）模型，检测Spy1的时空表达。Spy1表达在坐骨神经受压后逐渐升高，在第3天达到高峰。这种改变是由于SNC后轴突和雪旺细胞中Spy1的表达增加。Spy1表达与坐骨神经损伤后雪旺细胞增殖密切相关。此外，Spy1主要定位于受压节段的轴突，但很少与GAP43共定位。这些结果提示，Spy1参与了坐骨神经损伤的病理过程反应，并可能与雪旺细胞增殖和轴突再生有关。
As a novel cell cycle protein, Spy1 enhances cell proliferation, promotes the G1/S transition as well as inhibits apoptosis in response to UV irradiation. Spy1 levels are tightly regulated during mammary development, and overexpression of Spy1 accelerates tumorigenesis in vivo. But little is known about the role of Spy1 in the pathological process of damage and regeneration of the peripheral nervous system. Here we established a rat sciatic nerve crush (SNC) model to examine the spatiotemporal expression of Spy1. Spy1 expression was elevated gradually after sciatic nerve crush and peaked at day 3. The alteration was due to the increased expression of Spy1 in axons and Schwann cells after SNC. Spy1 expression correlated closely with Schwann cells proliferation in sciatic nerve post injury. Furthermore, Spy1 largely localized in axons in the crushed segment, but rarely co-localized with GAP43. These findings suggested that Spy1 participated in the pathological process response to sciatic nerve injury and may be associated with Schwann cells proliferation and axons regeneration.