Whole-Cell Patch-Clamp Recording
Whole-Cell Patch-Clamp Recording
复制标题
全细胞膜片钳记录
DOI：
--
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
W. Thoreson
中科院分区：
文献类型：
--
作者：
M. J. Hook;W. Thoreson
神经系统中的信号是由单个神经元膜上电压的变化来调节的。了解这些生物电信号-它们的离子基础、调制、信息内容、对细胞间通信的贡献，以及它们编码感觉输入和行为输出的方式-是当代神经科学研究的基本任务之一。已经开发出各种方法，使研究人员能够测量和操纵单个神经元和神经元网络的生物电--这是一系列统称为“电生理学”的实验方法。在这一章中，我们描述了全细胞膜片钳记录，这是一种电生理学技术，使研究人员能够记录膜电压，并通过使用电压钳技术，记录这些电压背后的离子电流。我们概述了全细胞记录的背景和原理，并讨论了这些实验所需的基本设备，包括空气台、显微镜、法拉第笼、微操作器、记录电极、放大器和其他电子设备、解决方案和记录吸管。我们还描述了成功的全细胞记录技术，包括穿孔补片技术的讨论，并注意到潜在的陷阱和质量控制问题，这些是获得高质量可发表数据的关键。
Signaling in the nervous system is mediated by changes in voltage across the membranes of individual neurons. Understanding these bioelectrical signals—their ionic basis, modulation, information content, contributions to intercellular communication, and ways in which they encode sensory input and behavioral output—is one of the fundamental tasks of contemporary neuroscience research. A variety of approaches have been developed that enable researchers to measure and manipulate bioelectricity of individual neurons and networks of neurons—a family of experimental approaches collectively termed “electrophysiology.” In this chapter, we describe whole-cell patch-clamp recording, an electrophysiological technique that enables researchers to record membrane voltages and, by using voltage-clamp techniques, the ionic currents underlying those voltages. We provide an overview of the background and principles of whole-cell recording as well as a discussion of the basic complement of equipment including air tables, microscopes, Faraday cages, micromanipulators, recording electrodes, amplifiers and other electronics, solutions, and recording pipettes needed for these experiments. We also describe techniques for successful whole-cell recording, including a discussion of perforated patch techniques, and note potential pitfalls and issues of quality control that are key for obtaining high-quality publishable data.
锥带突触的量子振幅可以通过胞质谷氨酸的变化来调节。
影响因子：
2.2
作者：
Bartoletti,TheodoreM;Thoreson,WallaceB
通讯作者：
Thoreson,WallaceB
影响因子：
2.5
作者：
W. Thoreson;E. J. Bryson;K. Rábl
通讯作者：
W. Thoreson;E. J. Bryson;K. Rábl