Saccharomyces cerevisiae ISU1 and ISU2:: Members of a well-conserved gene family for iron-sulfur cluster assembly
Saccharomyces cerevisiae ISU1 and ISU2:: Members of a well-conserved gene family for iron-sulfur cluster assembly
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DOI：
10.1006/jmbi.1999.3294
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发表时间：
1999-12-10
影响因子：
5.6
通讯作者：
Culotta, VC
中科院分区：
文献类型：
--
作者：
Garland, SA;Hoff, K;Culotta, VC
最近在细菌和真核生物中的研究已经鉴定了几个与含铁硫（Fe/S）簇蛋白的生物发生有关的新基因。本报告着重于面包酵母酿酒酵母的两个基因，ISU 1和ISU 2，它们编码细菌IscU和NifU的同源物，潜在的铁结合或簇组装蛋白。与涉及Fe/S蛋白组装的其他酵母基因一样，ISU 1或ISU 2的缺失导致线粒体内铁积累增加，[4Fe-4S]乌头酸酶活性丧失，以及缺乏细胞溶质铜/锌超氧化物歧化酶的细胞中氧化损伤的抑制。这两个基因在表达铁敏感转录因子Aft 1 p的激活等位基因的菌株中被诱导，表明它们受细胞铁状态的调节。使用针对大肠杆菌ISCU的抗体的免疫印迹研究表明，Isu 1 p和Isu 2 p主要定位于线粒体中，并且Isu 1 p是在所有测试的生长条件下表达的主要形式。的Isu蛋白在组装和/或修复的Fe/S簇的可能作用进行了讨论。(C)北京：科学出版社.
Recent studies in bacteria and eukaryotes have led to the identification of several new genes implicated in the biogenesis of iron-sulfur (Fe/S) cluster-containing proteins. This report focuses on two genes of bakers yeast Saccharomyces cerevisiae, ISU1 and ISU2, which encode homologues to bacterial IscU and NifU, potential iron-binding or cluster-assembly proteins. As with other yeast genes implicated in Fe/S protein assembly, deletion of either ISU1 or ISU2 results in increased accumulation of iron within the mitochondria, loss of activity of the [4Fe-4S] aconitase enzyme, and suppression of oxidative damage in cells lacking cytosolic copper/zinc superoxide dismutase. Both genes are induced in strains expressing an activated allele of Aft1p, the iron-sensing transcription factor, suggesting that they are regulated by the iron status of the cell. Immunoblotting studies using an antibody directed against Escherichia coli IscU reveal that both Isu1p and Isu2p are localized primarily in the mitochondria and that Isu1p is the predominant form expressed under all growth conditions tested. The possible role of the Isu proteins in the assembly and/or repair of Fe/S clusters is discussed. (C) 1999 Academic Press.