TUFT PROTEIN - ITS RELATIONSHIP WITH THE KERATINS AND THE DEVELOPING ENAMEL MATRIX
TUFT PROTEIN - ITS RELATIONSHIP WITH THE KERATINS AND THE DEVELOPING ENAMEL MATRIX
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DOI：
10.1007/bf02555967
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发表时间：
1989-06-01
影响因子：
4.2
通讯作者：
KIRKHAM, J
中科院分区：
文献类型：
--
作者：
ROBINSON, C;SHORE, RC;KIRKHAM, J
使用免疫细胞化学和蛋白质印迹技术中的多克隆抗体研究了源自成熟人类臼齿、人类皮肤角蛋白和正在发育的大鼠门牙牙釉质基质的簇蛋白之间的关系。毛簇蛋白和角蛋白的抗体与来自脱矿质发育中的牙釉质器官、人类皮肤和毛簇提取物的 Mr 范围 50-70 K 的蛋白质发生交叉反应。使用免疫细胞化学技术在超微结构水平上定位分泌性成釉细胞、牙釉质和角化上皮内的原位交叉反应位点。角蛋白抗体与细胞质张力丝和插入桥粒的抗体发生交叉反应。然而，簇蛋白抗体不与细胞骨架成分发生交叉反应，但产生高尔基体和分泌囊泡的标记。在大鼠足垫的颗粒层内也观察到用该抗体进行的标记。结论是簇蛋白含有成釉细胞的分泌产物，其可能代表其他上皮组织的不太特化的产物，可能与角蛋白有关。
The relationship among tuft protein derived from mature human molars, human skin keratins, and the developing enamel matrix of the rat incisor was investigated using polyclonal antibodies in immunocytochemical and Western blotting techniques. Antibodies to tuft protein and keratin cross-reacted with proteins in the Mr range 50-70 K from demineralized developing enamel organ, human skin, andn tuft extract. An immunocytochemical technique was used to locate the site of cross-reactivity in situ within secretory ameloblasts, enamel, and keratinized epithelium at the ultrastructural level. Antibodies to keratin cross-reacted with cytoplasmic tonofilaments and those inserted into desmosomes. Antibodies to tuft protein, however, did not cross-react with cytoskeletal components but produced labeling of the golgi and secretory vesicles. Labeling with this antibody was also observed within the stratum granulosum of the rat foot pad. It is concluded that tuft protein contains secretory products of the ameloblast that may represent a less specialized product of other epithelial tissues, perhaps related to the keratins.