Detection of oral bacteria in cardiovascular specimens
Detection of oral bacteria in cardiovascular specimens
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DOI：
10.1111/j.1399-302x.2008.00479.x
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发表时间：
2009-02-01
影响因子：
--
通讯作者：
Ooshima, T.
中科院分区：
文献类型：
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作者：
Nakano, K.;Nemoto, H.;Ooshima, T.
口腔细菌，包括致龋和牙周病原体，被认为是心血管疾病发展的病因学因素。为了确定这种关系，我们分析了口腔细菌种类在心血管标本中的分布，在接受研究后，分析了203例连续患者，其中82例主动脉瓣标本，35例二尖瓣标本和86例主动脉瘤壁标本，其中16例含有主动脉瘤血栓组织。此外，从接受心脏瓣膜置换术或主动脉瘤切除术的同一组患者中共收集了58个牙菌斑样本。分别从患者的心血管组织和牙菌斑中提取细菌DNA，采用种特异性聚合酶链反应（PCR）方法检测6种口腔链球菌和6种牙周细菌的检出率，结果显示，在心血管组织中检出率最高的是变形链球菌，其次是伴放线菌聚集杆菌。至于接受心血管手术的患者的牙菌斑标本，大多数测试牙周炎相关物种以及口腔链球菌检测频率高。此外，S.牙菌斑标本中S.变形链球菌的检出率为78%，显著高于其他菌种。mutans和A.放线菌共生菌与菌血症有关，可能是心血管疾病发生的致病因素。
Oral bacteria, including cariogenic and periodontal pathogens, are thought to be etiological factors in the development of cardiovascular diseases. To define this relationship, we analyzed the distribution of oral bacterial species in cardiovascular specimens.Following acceptance into the study, 203 consecutive patients were analyzed, from whom 82 aortic valve specimens, 35 mitral valve specimens, and 86 aortic aneurysmal wall specimens, of which 16 contained aneurysmal thrombus tissues, were obtained. In addition, a total of 58 dental plaque specimens were collected from the same group of patients who underwent heart valve replacement or removal of aortic aneurysms. Bacterial DNA was extracted from both cardiovascular tissues and dental plaque in those cases and then species-specific polymerase chain reaction assays were used to analyze the occurrences of six oral streptococcal and six periodontal bacterial species.Streptococcus mutans was the most frequently detected species in the cardiovascular specimens, followed by Aggregatibacter actinomycetemcomitans. As for dental plaque specimens from patients who underwent cardiovascular operations, most of the tested periodontitis-related species as well as oral streptococci were detected at high frequencies. Furthermore, the positive rate of S. mutans in cardiovascular specimens from patients whose dental plaque specimens were also positive for S. mutans was 78%, which was significantly higher than any other tested species when the same analysis was performed.Our results suggest that specific oral bacterial species, such as S. mutans and A. actinomycetemcomitans, are related to bacteremia and may be etiologic factors for the development of cardiovascular diseases.