Exercise training attenuates septic responses in conscious rats
Exercise training attenuates septic responses in conscious rats
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DOI：
10.1249/mss.0b013e31802d11c8
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发表时间：
2007-03-01
期刊：
影响因子：
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通讯作者：
Lin, Chia-Chih
中科院分区：
文献类型：
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作者：
Chen, Hsing I.;Hsieh, Shu-Ya;Lin, Chia-Chih
目的：观察运动训练对内毒素所致大鼠动脉压、心率、血细胞、生化指标、血浆亚硝酸盐/硝酸盐、甲基胍（MG）、促炎细胞因子及心、肝、肺病理的影响。方法：24只10周龄雄性Wistar-Kyoto大鼠，体重320-350 g，随机分为两组。运动训练组（Tr; N = 12）接受4 wk的运动训练。对照组（Con）被放置在跑步机上，并保持久坐相同的时间段。通过静脉内（i. v.）内毒素（LPS; 10 mg（.）kg（-1））20 min后观察72 h。股动脉插管，监测动脉压和HR。血液样品收集前1小时和LPS输注后的不同时间。我们测定了血浆亚硝酸盐/硝酸盐、MG、白色血细胞、中性粒细胞、淋巴细胞、红细胞、血尿素氮、肌酐（Cr）、天冬氨酸转氨酶、丙氨酸转氨酶、乳酸脱氢酶、肌酸磷酸激酶、淀粉酶、脂肪酶、肿瘤坏死因子（α）和白细胞介素-1（β）。实验结束后取心、肝、肺组织进行病理学检查和评价。结果：Tr组的基础动脉压、HR、MG、中性粒细胞和Cr水平低于Con组。运动训练减弱了LPS诱导的血细胞减少。LPS给药后，Con组的硝酸盐/亚硝酸盐、MG、生化因子和促炎细胞因子的血浆水平高于Tr组。病理检查和评估显示，心脏，肝脏和肺损伤在Con组比Tr组更严重。结论：运动训练可减轻脓毒症患者的脓毒症反应，保护器官免受脓毒症的损害。
Purpose: To evaluate the effects of exercise training on the changes induced by endotoxin in arterial pressure, heart rate (HR), blood cells, biochemical factors, plasma nitrite/nitrate, methyl guanidine (MG), proinflammatory cytokines, and pathology of the heart, liver, and lung. Methods: Twenty-four 10-wk-old male Wistar-Kyoto rats weighing 320-350 g were randomly assigned into two groups. The exercise-trained group (Tr; N = 12) received exercise training for 4 wk. The control (Con) group was placed on the treadmill and remained sedentary for the same time period. Endotoxemia was induced by intravenous (i.v.) infusion of lipopolysaccharide (LPS; 10 mg(.)kg(-1)) for 20 min, after which the animals were observed for 72 h. The femoral artery was cannulated to monitor arterial pressure and HR. Blood samples were collected 1 h before and at various times after LPS infusion. We determined plasma nitrite/nitrate, MG, white blood cells, neutrophils, lymphocytes, red blood cells, blood urea nitrogen, creatinine (Cr), aspartate aminotransferase, alanine aminotransferase, lactic acid dehydrogenase, creatine phosphokinase, amylase, lipase, tumor necrosis factor(alpha), and interleukin-1(beta). The heart, liver, and lung were taken for pathological examination and assessment after the experiment. Results: The Tr group had lower basal levels of arterial pressure, HR, MG, neutrophils, and Cr than the Con group. Exercise training attenuated the LPS-induced decreases in blood cells. After LPS administration, plasma levels of nitrate/nitrite, MG, biochemical factors, and proinflammatory cytokines in the Con group were higher than in the Tr group. Pathological examination and assessment revealed that cardiac, hepatic, and pulmonary injury were more severe in the Con group than in the Tr group. Conclusions: Exercise training attenuates septic responses and protects organs from damage in sepsis.