THE DISTRIBUTION OF HISTOCOMPATIBILITY ANTIGENS ON T AND B CELLS IN THE GUINEA PIG
THE DISTRIBUTION OF HISTOCOMPATIBILITY ANTIGENS ON T AND B CELLS IN THE GUINEA PIG
复制标题
豚鼠T细胞和B细胞组织相容性抗原的分布
DOI：
10.1097/00007890-197308000-00008
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发表时间：
1973
期刊：
影响因子：
6.2
通讯作者：
I. Green
中科院分区：
文献类型：
--
作者：
E. Shevach;D. Rosenstreich;I. Green
摘要 用交叉免疫2株和13株动物制备的同种抗血清检查了豚鼠不同淋巴细胞群上组织相容性（H）抗原的分布。这些同种抗血清仅杀死 30-40% 的淋巴结细胞，而那些被同种抗血清杀死后仍然存活的细胞在携带免疫球蛋白的 B 淋巴细胞中明显减少。抗2血清的吸收研究表明，淋巴结细胞的同种异体抗原含量是胸腺细胞的7-8倍，而L2C白血病细胞（纯恶性B细胞群）的同种异体抗原含量是胸腺细胞的25-30倍。此外，通过用抗T细胞血清和C处理淋巴结细胞制备的纯B淋巴结细胞在同种抗血清的吸收方面比正常淋巴结细胞更有效。最后，用同种抗血清和 C 处理后仍然存活的淋巴结细胞未能对内毒素（一种 B 细胞有丝分裂原）做出反应，而这些相同的细胞对植物血凝素（一种 T 细胞有丝分裂原）做出正常反应。这些结果表明，与 T 淋巴细胞相比，B 淋巴细胞上的 H 抗原含量有所增加。由于 H 抗原在 B 细胞和 T 细胞之间的合作中发挥作用，因此有关 H 抗原在这些细胞上分布的信息可能对免疫反应中细胞间相互作用具有重要意义。
SUMMARY The distribution of histocompatibility (H) antigens on different lymphocyte populations in the guinea pig was examined with alloantisera prepared by cross immunizing strain 2 and strain 13 animals. These alloantisera killed only 30-40% of lymph node cells, and those cells that remain alive after killing by the alloantisera are markedly depleted in immunoglobulin-bearing B lymphocytes. Absorption studies of the anti-2 serum demonstrated that the alloantigen content of lymph node cells was 7-8 times that of thymocytes, while the alloantigen content of L2C leukemia cells, a pure population of malignant B cells, was 25-30 times that of thymocytes. Furthermore, pure B lymph node cells, prepared by treating lymph node cells with an anti-T cell serum and C, were more efficient than normal lymph node cells in the absorption of the alloantisera. Finally, those lymph node cells which remain alive after treatment with the alloantisera and C failed to respond to endotoxin, a B cell mitogen, whereas these same cells responded normally to phytohemagglutinin, a T cell mitogen. These results demonstrate an increased content of H antigen on the B lymphocyte compared to the T lymphocyte. Since H antigens play a role in the cooperation between B and T cells, information as to the distribution of H antigens on these cells may have important implications for cell to cell interaction in the immune response.