Transcriptomic and functional analyses uncover the regulatory role of lncRNA000170 in tomato multicellular trichome formation
Transcriptomic and functional analyses uncover the regulatory role of lncRNA000170 in tomato multicellular trichome formation
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转录组和功能分析揭示了lncRNA000170在番茄多细胞毛状体形成中的调节作用
DOI：
10.1111/tpj.14902
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发表时间：
2020-07-15
期刊：
影响因子：
7.2
通讯作者：
Yang, Changxian
中科院分区：
文献类型：
--
作者：
Liao, Xiaoli;Wang, Junqiang;Yang, Changxian
毛状体是一种普遍的特殊结构，起源于几乎所有的陆地植物。虽然在许多植物中已经发现了大量的长非编码RNA(LncRNAs)，但LncRNAs在毛状体形成中的作用仍不清楚。在此，我们在绵毛突变体LA3560(Wo)及其非羊毛分离物(WT)的幼茎中总共鉴定了1303个lncRNAs。其中86个lncRNAs明显上调，110个lncRNAs表达下调。通过定量逆转录聚合酶链式反应，我们确定了7个lncRNAs在LA3560的茎毛状体和其他几个组织中高表达，包括lncRNA000746、lncRNA000170、lncRNA000277、lncRNA000774、lncRNA000756、lncRNA000100和lncRNA000898。转基因实验表明，过表达lncRNA000170抑制了成年转基因植株下部茎上I型毛的形成。我们进一步确定了lncRNA000170是从Solyc10g006360的互补链转录而来的，lncRNA000170可以在其过表达系和羊毛突变体中诱导表达。Solyc10g006360过表达还导致I型毛状体减少。此外，在LncRNA000170高表达系中，几种毛状体调节因子，如Wo、H、SlCycB2和SlCycB3显著下调。这些发现表明，lncRNA000170可能参与了这些毛状体调节因子介导的调控途径。
Trichomes are universal specific structures originating from nearly all terrestrial plants. Although quantities of long non-coding RNAs (lncRNAs) have been identified in many plant species, the role of lncRNAs in trichome formation still remains unknown. Here, we identified a total of 1303 lncRNAs in the young stems of woolly mutant LA3560 (Wo) and its non-woolly segregants (WT). Out of these lncRNAs, 86 lncRNAs were obviously upregulated inWoand 110 lncRNAs were downregulated. We determined that seven lncRNAs were highly expressed in stem trichomes compared to trichome-free stems and several other tissues of LA3560 by a quantitative reverse transcriptase-polymerase chain reaction, including lncRNA000746, lncRNA000170, lncRNA000277, lncRNA000774, lncRNA000756, lncRNA000100, and lncRNA000898. Transgenic experiments revealed that overexpression of lncRNA000170 inhibited type I trichome formation on the lower stems of the adult transgenic plants. We further determined that lncRNA000170 was transcribed from the complementary strand of Solyc10g006360, for which expression can be induced by lncRNA000170 in its overexpression lines and woolly mutants. Solyc10g006360 overexpression also caused type I trichome decrease. In addition, several trichome regulators, such asWo,H,SlCycB2, andSlCycB3, were markedly downregulated in lncRNA000170 overexpression lines. These findings demonstrate that lncRNA000170 may be involved in the regulatory pathway mediated by these trichome regulators.