ISOLATION AND PRELIMINARY GENETIC AND BIOCHEMICAL CHARACTERIZATION OF TEMPERATURE-SENSITIVE MUTANTS OF REOVIRUS
ISOLATION AND PRELIMINARY GENETIC AND BIOCHEMICAL CHARACTERIZATION OF TEMPERATURE-SENSITIVE MUTANTS OF REOVIRUS
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DOI：
10.1016/0042-6822(69)90217-7
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发表时间：
1969-01-01
期刊：
影响因子：
3.7
通讯作者：
JOKLIK, WK
中科院分区：
文献类型：
--
作者：
FIELDS, BN;JOKLIK, WK
已分离出一组呼肠孤病毒3型的温度敏感突变体。使用的诱变剂是亚硝酸，亚硝基胍，和proflavin.35突变体进行了测试，他们的重组能力后成对感染。已检测到五个重组组。29个突变体属于一个组，4个属于另一个组，3个突变体与其他突变体有效重组。振荡频率为零或超过2%。这一结果支持了呼肠孤病毒基因组由多个片段组成的化学证据。根据这一标准对突变体的分类与从重组试验中推导出的分类完全一致。
A set of temperature-sensitive mutants of reovirus type 3 has been isolated. The mutagens used were nitrous acid, nitrosoguanidine, and proflavine.Thirty-five mutants have been tested for their ability to recombine following pairwise infection. Five recombination groups have been detected. Twenty-nine mutants fall into one group, four into another, and three mutants recombine efficiently with every other mutant tested. Recombination frequencies are either zero or in excess of 2%. This result supports chemical evidence that the genome of reovirus consists of a number of segments.The mutants have been tested for their ability to induce the formation of virus-specific RNA at the nonpermissive temperature. The classification of mutants according to this criterion agrees exactly with that deduced from recombination tests.