Effect of isoorientin on intracellular antioxidant defence mechanisms in hepatoma and liver cell lines
Effect of isoorientin on intracellular antioxidant defence mechanisms in hepatoma and liver cell lines
复制标题
异荭草素对肝癌和肝细胞系细胞内抗氧化防御机制的影响
DOI：
10.1016/j.biopha.2016.04.025
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发表时间：
2016-07-01
影响因子：
7.5
通讯作者：
Liu, Xuebo
中科院分区：
文献类型：
--
作者：
Yuan, Li;Wang, Jing;Liu, Xuebo
异orientin（ISO）被认为是负责健康益处的最重要的类黄酮化合物之一，包括预防肝损伤以及抗氧化、抗炎和抗伤害活性。我们前期的研究表明，ISO通过增加细胞内ROS水平抑制肝癌细胞的增殖。有趣的是，ISO通过降低细胞内ROS水平来保护大鼠肝细胞免受过氧化氢诱导的氧化应激。为什么ISO在不同的生理和病理生理条件下对ROS的影响不同？本研究探讨了异硫氰酸酯（ISO）对人肝母细胞瘤细胞（HepG 2）、布法罗大鼠肝细胞（BRL-3A）和人肝癌细胞（HL-7702）线粒体呼吸链复合物和II相解毒酶活性的影响。结果显示，HepG 2细胞内ROS水平和呼吸链复合物蛋白表达均显著高于BRL-3A和HL-7702细胞（P < 0.01）。另外，ISO显著增加HepG 2细胞中的ROS水平（p < 0.01），而在BRL-3A和HL-7702细胞中未发现显著性。ISO可降低HepG 2细胞呼吸链复合物蛋白表达、II相解毒酶活性和GSH含量（p < 0.01），但在一定范围内可提高BRL-3A和HL-7702细胞呼吸链复合物蛋白表达、II相解毒酶活性和GSH含量。所有这些结果表明，第一次，ISO具有显着的肝保护作用，这可能是通过呼吸链复合物和II相解毒酶活性介导的。(C)2016年由Elsevier Masson SAS出版。
Isoorientin (ISO) is considered one of the most important flavonoid-like compounds responsible for health benefits, including the prevention of liver damage as well as antioxidant, anti-inflammatory, and anti-nociceptive activities. Our previous study showed that ISO inhibited the proliferation of hepatoma cells through increasing intracellular ROS levels. Interestingly, ISO protects rat liver cells against hydrogen peroxide-induced oxidation stress by decreasing intracellular ROS levels. Why are there different effects of ISO on ROS in different physiological and pathophysiological circumstances? The present study investigated the effect of ISO on mitochondrial respiratory chain complexes and phase II detoxifying enzyme activities in human hepatoblastoma cancer cells (HepG2), buffalo rat liver cells (BRL-3A) and human liver cancer cells (HL-7702). The results showed that intracellular ROS levels and the protein expression of the respiratory chain complexes was significantly (p < 0.01) higher in the HepG2 cells than in the BRL-3A and HL-7702 cells. Additionally, ISO notably (p < 0.01) increased ROS levels in the HepG2 cells, while no significance was found in the BRL-3A and HL-7702 cells. Furthermore, in the HepG2 cells, the protein expression of the respiratory chain complexes and the phase II detoxifying enzyme activities and GSH content were decreased by ISO (p < 0.01), while ISO, in a certain range, enhanced the expression of the protein complexes and the phase II detoxifying enzyme activities and GSH content in BRL-3A and HL-7702 cells. All of these results demonstrated, for the first time, that ISO possesses a notable hepatoprotective effect, which might be mediated through the respiratory chain complexes and phase II detoxifying enzyme activities. (C) 2016 Published by Elsevier Masson SAS.