A possible role for cold insoluble globulin in chemotactic factor mediated polymorphonuclear leukocyte adherence to plastic.
A possible role for cold insoluble globulin in chemotactic factor mediated polymorphonuclear leukocyte adherence to plastic.
复制标题
冷不溶性球蛋白在趋化因子介导的多形核白细胞粘附到塑料中的可能作用。
DOI：
10.1016/0006-291x(82)91134-2
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发表时间：
1982
影响因子：
3.1
通讯作者：
L. Culp
中科院分区：
文献类型：
--
作者：
P. Spagnuolo;M. Lederman;J. Schiltz;J. Ellner;L. Culp
我们研究了纤连蛋白在趋化因子刺激中性粒细胞粘附塑料中的潜在作用。人纤连蛋白的单克隆抗体显着减少趋化肽刺激的粘附，但没有减少粘附刺激佛波醇肉豆蔻酸酯或聚集刺激的任何代理。趋化肽刺激中性粒细胞也与免疫荧光或放射性标记胶原蛋白结合检测到的细胞表面纤连蛋白的丢失有关。这些数据表明，趋化肽刺激中性粒细胞释放Fn和Fn介导的中性粒细胞的附着到塑料表面。
We examined the potential role of fibronectin in chemotactic factor stimulation of neutrophil adherence to plastic. Monoclonal antibody to human fibronectin significantly reduced chemotactic peptide stimulation of adherence but did not reduce adherence stimulated by phorbol myristate acetate or aggregation stimulated by either agent. Stimulation of neutrophils by chemotactic peptide was also associated with loss of cell surface fibronectin detected by immunofluorescence or binding of radiolabeled collagen. These data suggest that chemotactic peptides stimulate neutrophils to release Fn and that Fn mediates the attachment of neutrophils to plastic surfaces.