Collection, isolation and culturing strategies for Oxyrrhis marina
Collection, isolation and culturing strategies for Oxyrrhis marina
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DOI：
10.1093/plankt/fbq161
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发表时间：
2011-04-01
影响因子：
2.1
通讯作者：
Montagnes, David J. S.
中科院分区：
文献类型：
--
作者：
Lowe, Chris D.;Martin, Laura E.;Montagnes, David J. S.
异养海洋鞭毛虫Oxyrris marina是一种受欢迎且易驯化的模式生物，在潮间带环境中很常见，在实验室中维护相对简单。在这份报告中，基于我们收集和培养类似于400个菌株的经验，我们提供了一个量化的方法，即从环境中定位和分离并随后在培养中保持的海洋假单胞菌有多容易。此外，我们提供了一个简短的文献回顾与养殖，特别强调了广泛的生理耐受性和广泛的饮食范围的这种生物。最后，鉴于人们对海洋假单胞藻的遗传学和基因组学以及异养原生生物的兴趣与日俱增，我们重点介绍了培养异养生物用于“非基因组”应用的现有策略，并总结了我们用于海洋假单胞藻基因组和蛋白质组学研究的具体培养方法。在此背景下，我们概述了Droop的无菌培养的简化版本，并提供了在没有其他真核生物和活的原核生物的情况下，使用热灭活的大肠杆菌作为简单而健壮的食物来源来培养海洋对虾的详细信息。
The heterotrophic marine flagellate Oxyrrhis marina is a popular and tractable model organism, being common in the intertidal environment and relatively straight forward to maintain in the laboratory. In this report, based on our experience of collecting and culturing similar to 400 isolates, we provide a quantification of how "easy" O. marina is to locate and isolate from the environment and subsequently maintain in culture. In addition, we provide a brief review of the literature pertaining to O. marina culturing, stressing in particular the broad physiological tolerance and a wide diet range of this organism. Finally, in the light of the increasing interest in the genetics and genomics of O. marina, and heterotrophic protists more generally, we highlight existing strategies for culturing heterotrophs for "-omic" applications and summarize our specific culturing approaches for the genomic and proteomic studies of O. marina. In this context, we outline a simplified version of Droop's medium for axenic culturing and offer details on the use of heat-killed Escherichia coli as a simple and robust food source for culturing O. marina in the absence of other eukaryotes and live prokaryotes.