Cross-validation of Input Functions Obtained by H215O PET Imaging of Rat Heart and a Blood Flow-through Detector
Cross-validation of Input Functions Obtained by H215O PET Imaging of Rat Heart and a Blood Flow-through Detector
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大鼠心脏 H215O PET 成像和血流检测器获得的输入函数的交叉验证
DOI：
10.1007/s11307-011-0511-5
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发表时间：
2012
影响因子：
3.1
通讯作者：
A. Roivainen
中科院分区：
文献类型：
--
作者：
N. Kudomi;H. Sipilä;Anu Autio;V. Oikonen;H. Liljenbäck;M. Tarkia;J. Laivola;J. Johansson;M. Teräs;A. Roivainen
目的15 O标记水（H215 O）正电子发射断层扫描（PET）有助于临床和小动物血流的可视化和定量。血流的量化需要输入函数，其通常通过测量在PET扫描期间抽取的动脉血中的放射性来获得。然而，这种方法并不总是可行的，因为大量的血液采样可能会影响被测量的生理过程。本研究的目的是开发和交叉验证两种方法，即，血液和基于图像的方法获得输入功能的血流研究从大鼠H215 O PET.MethodsThe研究材料包括两个独立的组的大鼠。第1组大鼠在静息条件下通过高分辨率研究断层扫描PET照相机成像两次，用于测试-再测试研究（n = 4），第2组大鼠在有和没有腺苷输注的情况下成像，用于静息-应激研究（n = 4）。在第1组中，在成像过程中使用新的流通检测器测量动脉血中的放射性浓度，并获得基于血液的输入函数。使用来自左心室和心肌区域的时间-活动曲线来估计基于图像的输入函数。为了验证这两种输入函数的方法，心肌血流量（MBF）和脑血流量（CBF）进行了计算，并进行了重复性（测试-再测试研究）和变化（休息-应力研究）的方法进行了测试。结果血液和图像为基础的输入函数是相似的，相应的CBF值相差只有-6.9 ± 8.1%。在重测研究中，MBF和CBF均显示出良好的重复性，在静息-应激研究中，腺苷显著增加MBF和CBF（P = 0.035）和CBF结论利用H215 O PET显像测量大鼠股动脉的输入函数和估计大鼠H215 O PET显像的输入函数是可行的，从而便于评估PET图像中可见的器官中的血流。
PurposePositron emission tomography (PET) with 15O-labeled water (H215O) facilitates the visualization and quantification of blood flow in clinical investigations and also in small animals. The quantification of blood flow requires an input function, which is generally obtained by measuring radioactivity in arterial blood withdrawn during PET scanning. However, this approach is not always feasible, because abundant blood sampling may affect the physiological process being measured. The purpose of the present study was to develop and cross-validate two methods, namely, a blood- and an image-based method for obtaining the input function for blood flow studies from rat H215O PET.MethodsThe study material consisted of two separate groups of rats. Group 1 rats were imaged twice by a high-resolution research tomograph PET camera at resting condition for a test–retest study (n = 4), and group 2 rats were imaged with and without adenosine infusion for a rest–stress study (n = 4). In group 1, radioactivity concentration in arterial blood was measured with a new flow-through detector during imaging and a blood-based input function was obtained. The image-based input function was estimated using time-activity curves from the left ventricle and myocardial regions. To validate the two input function methods, myocardial blood flow (MBF) and cerebral blood flow (CBF) were computed, and the methods were tested for reproducibility (test–retest study) and changes (rest–stress study).ResultsThe blood- and image-based input functions were similar, and the corresponding CBF values differed only by −6.9 ± 8.1%. In the test–retest study, both MBF and CBF showed good reproducibility, and in the rest–stress study, adenosine significantly increased both MBF (P = 0.035) and CBF (P = 0.029), compared with the resting condition.ConclusionIt is possible both to measure the input function from rat arteria femoralis during H215O PET imaging and to estimate the input function from rat H215O PET images, thereby facilitating the assessment of blood flow in organs visible in PET images.
影响因子：
3.1
作者：
Laforest, R;Sharp, TL;Welch, MJ
通讯作者：
Welch, MJ